POLR2B 抗体 (N-Term)
(1 reference)Quick Overview for POLR2B 抗体 (N-Term) (ABIN2855420)
抗原
See all POLR2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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交叉反应
- 大鼠, 猕猴, 牛
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交叉反应 （详细）
- Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)
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产品特性
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Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
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亚型
- IgG
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anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 1-260) antibody
POLR2B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 805-854) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Pig, 非洲爪蟾, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) antibodyPOLR2B 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibodyPOLR2B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (AA 119-168) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (N-Term) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 非洲爪蟾, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (Middle Region) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 非洲爪蟾, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B) (Middle Region) antibodyPOLR2B 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
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说明
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Positive Control: H1299 , BCL-1
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.65 mg/mL
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缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." in: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).
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: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." in: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).
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- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
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别名
- RPB2
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背景
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This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
Cellular Localization: Nucleus -
分子量
- 134 kDa
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基因ID
- 5431
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途径
- Regulatory RNA Pathways, DNA Damage Repair
抗原
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