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PHGDH 抗体 (N-Term)

Name: PHGDH 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2855237
Availability: OutOfStock

This anti-PHGDH antibody is a 兔多克隆 antibody detecting PHGDH in WB, IHC (p), IF, IP, IHC (fro) 和 ICC. Suitable for 人.

WB Image Sample(30 μg of whole cell lysate) A:293T B:A431, C:HeLa S3, 7. (PHGDH 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH, antibody at 1:500 dilution. (PHGDH 抗体 (N-Term))

IHC

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH, antibody at 1:500 dilution. (PHGDH 抗体 (N-Term))

IP Image Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term, Western blot analysis was performed using PHGDH antibody [N1N2], N-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (PHGDH 抗体 (N-Term))

WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 7. (PHGDH 抗体 (N-Term))

IHC-P Image PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis. (PHGDH 抗体 (N-Term))

IHC

6 images

产品编号 ABIN2855237

发货至: 中国

Quick Overview for PHGDH 抗体 (N-Term) (ABIN2855237)

抗原

See all PHGDH 抗体

PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PHGDH antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

质量等级

KO Validated

Discover our top product PHGDH Primary Antibody

抗原表位
15

8

5

5

4

2

1

1

1

1

1

1

1

1

1

1
N-Term

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

亚型

IgG
anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB, ELISA, IHC (p), IF, IP 宿主: 小鼠 Monoclonal 4A3-1D6 unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 264-533) antibody
PHGDH 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168) antibody
PHGDH 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 2-251) antibody
PHGDH 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibody
PHGDH 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 1-533) antibody
PHGDH 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (N-Term) antibody
PHGDH 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 249-277) antibody
PHGDH 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20883 unconjugated

anti-phosphoglycerate Dehydrogenase (PHGDH) (AA 140-168), (N-Term) antibody
PHGDH 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20882 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa
Validation: KO/KD

限制

仅限研究用
状态

Liquid

浓度

0.87 mg/mL

缓冲液

0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

别名

phosphoglycerate dehydrogenase

背景

3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

分子量

57 kDa

基因ID

26227

UniProt

O43175

途径

Metabolism of Steroid Hormones and Vitamin D, Warburg Effect