HMGB1 抗体
Quick Overview for HMGB1 抗体 (ABIN2854997)
抗原
See all HMGB1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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交叉反应
- 人, 小鼠, Pig, 大鼠, 斑马鱼
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产品特性
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Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T
Validation: KO/KD, Orthogonal, Overexpression
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.46 mg/mL
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缓冲液
- 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).
: "N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a histone deacetylase inhibitor, induces the release of nuclear HMGB1 and modifies ROS levels in HeLa cells." in: Oncotarget, Vol. 9, Issue 70, pp. 33368-33381, (2018) (PubMed).
: "Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs." in: Molecular therapy. Methods & clinical development, Vol. 3, pp. 16041, (2016) (PubMed).
: "Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis." in: Frontiers in cellular neuroscience, Vol. 9, pp. 485, (2016) (PubMed).
: "
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N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- high mobility group box 1
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背景
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DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Cellular Localization: Nucleus -
分子量
- 25 kDa
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基因ID
- 3146
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UniProt
- P09429
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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