我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

HMGB1 抗体

Name: HMGB1 抗体
Brand: antibodies-online
SKU: ABIN2854997
Availability: OutOfStock

This anti-HMGB1 antibody is a 兔多克隆 antibody detecting HMGB1 in WB, IHC, IF, ICC, IHC (p), IHC (wm) 和 IHC (ff). Suitable for 人. This Primary Antibody has been cited in 4+ publications.

WB Image HMGB1 antibody detects HMGB1 protein by western blot analysis. (HMGB1 抗体)

ICC/IF Image HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. (HMGB1 抗体)

IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis. (HMGB1 抗体)

IHC

IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis. (HMGB1 抗体)

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using HMGB1, antibody at 1:500 dilution. (HMGB1 抗体)

IHC

IHC-P Image HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis. (HMGB1 抗体)

IHC

Intracellular location of HMGB1 in HeLa cells at the basal state and in response to treatment with LPS, VPA and OH-VPA(A) HMGB1 protein (red) increased in cells that received any of the treatments. (HMGB1 抗体)

IF (cc)

12 images

(4 references)

产品编号 ABIN2854997

发货至: 中国

Datasheet as PDF

Quick Overview for HMGB1 抗体 (ABIN2854997)

抗原

See all HMGB1 抗体

HMGB1 (High Mobility Group Box 1 (HMGB1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This HMGB1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm)), Immunohistochemistry (Free Floating) (IHC (ff))

质量等级

KO Validated

Discover our top product HMGB1 Primary Antibody

交叉反应

人, 小鼠, Pig, 大鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.

亚型

IgG
anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibody
HMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 小鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal C4 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 124-154) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T
Validation: KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.46 mg/mL

缓冲液

1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Sixto-López, Rosales-Hernández, Contis-Montes de Oca, Fragoso-Morales, Mendieta-Wejebe, Correa-Basurto, Abarca-Rojano, Correa-Basurto: "N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).

Contis-Montes de Oca, Rodarte-Valle, Rosales-Hernández, Abarca-Rojano, Rojas-Hernández, Fragoso-Vázquez, Mendieta-Wejebe, Correa-Basurto, Vázquez-Moctezuma, Correa-Basurto: "N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a histone deacetylase inhibitor, induces the release of nuclear HMGB1 and modifies ROS levels in HeLa cells." in: Oncotarget, Vol. 9, Issue 70, pp. 33368-33381, (2018) (PubMed).

Bhatia, OBrien, Chen, Wong, Garner, Woodley, Li: "Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs." in: Molecular therapy. Methods & clinical development, Vol. 3, pp. 16041, (2016) (PubMed).

Juranek, Daffu, Wojtkiewicz, Lacomis, Kofler, Schmidt: "Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis." in: Frontiers in cellular neuroscience, Vol. 9, pp. 485, (2016) (PubMed).
抗原

HMGB1 (High Mobility Group Box 1 (HMGB1))

别名

high mobility group box 1

背景

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Cellular Localization: Nucleus

分子量

25 kDa

基因ID

3146

UniProt

P09429

途径

p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome