EIF2A 抗体 (Center)
Quick Overview for EIF2A 抗体 (Center) (ABIN2854984)
抗原
See all EIF2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Center
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交叉反应
- 猕猴
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交叉反应 (详细)
- Rhesus Monkey (100 %)
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产品特性
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Rabbit Polyclonal antibody to eIF2 alpha (eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa)
eIF2 alpha antibody [N1C3] -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human eIF2 alpha. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
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说明
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Positive Control: H1299 , Raji , *rat neuron , *HeLa , *N2a ,
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.31 mg/mL
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缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130599, (2016) (PubMed).
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Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130599, (2016) (PubMed).
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- eIF2 alpha
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背景
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The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]
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分子量
- 36 kDa
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基因ID
- 1965
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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