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Nanog 抗体

Name: Nanog 抗体
Brand: antibodies-online
SKU: ABIN2854880
Availability: OutOfStock

Cited in 6+ publications. This anti-Nanog antibody is a 兔多克隆 antibody detecting Nanog in WB, ELISA, IF, IHC, ICC, IHC (p), IP 和 IHC (fro). Suitable for 人.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:3000 (Nanog 抗体)

WB Image Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE antibody diluted at 1:3000 (Nanog 抗体)

ICC/IF Image NANOG antibody [N3C3] detects NANOG protein at nucleus by immunofluorescent analysis. (Nanog 抗体)

WB Image Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE antibody diluted at 1:3000 (Nanog 抗体)

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using NANOG, antibody at 1:400 dilution. (Nanog 抗体)

ELISA

IP Image Immunoprecipitation of NANOG protein from NT2D1 whole cell extracts using 5 μg of NANOG antibody [N3C3], Western blot analysis was performed using NANOG antibody [N3C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent. (Nanog 抗体)

Thiostrepton suppresses tumor sphere- and colony-formation potentials of HCT-15 and HT-29 cells as well as the expression of factors involved in stemness maintenance. (Nanog 抗体)

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs). (Nanog 抗体)

Abnormal developmental gene induction in ESCs lacking Oct1. (Nanog 抗体)

Gene expression defects upon differentiation of Oct1 inducible-conditional ESCs. (Nanog 抗体)

Isolation of inner cell mass and derivation/characterization of embryonic stem cell lines. (Nanog 抗体)

IF (p)

Stem cell and receptor markers expressed in iPS87 cells. (Nanog 抗体)

IF (cc)

14 images

(6 references)

产品编号 ABIN2854880

发货至: 中国

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Quick Overview for Nanog 抗体 (ABIN2854880)

抗原

See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

人

宿主

兔

克隆类型

多克隆

标记

This Nanog antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

Ceratotherium simum, 人, 小鼠

产品特性

Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
Nanog antibody [N3C3]

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

亚型

IgG
anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E11 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HeLa nucleus
Validation: Orthogonal

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Shen, Kang, Shakya, Tabaka, Jarboe, Regev, Tantin: "Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, (2018) (PubMed).

Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

Marinho, Chailangkarn, Muotri: "Systematic optimization of human pluripotent stem cells media using Design of Experiments." in: Scientific reports, Vol. 5, pp. 9834, (2016) (PubMed).

Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

Singh, Chappell, Trost, Lin, Wang, Tang, Matlock, Weller, Wu, Zhao, Jin, Dalton: "Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells." in: Stem cell reports, Vol. 1, Issue 6, pp. 532-44, (2015) (PubMed).

Liu, Bain: "Arsenic inhibits hedgehog signaling during P19 cell differentiation." in: Toxicology and applied pharmacology, Vol. 281, Issue 3, pp. 243-53, (2014) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

Nanog homeobox

背景

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

Cellular Localization: Nucleus

分子量

35 kDa

基因ID

79923

UniProt

Q9H9S0

途径

Stem Cell Maintenance