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Nanog 抗体

This anti-Nanog antibody is a 兔 多克隆 antibody detecting Nanog in WB, ELISA, IF, IHC, ICC, IHC (p), IP 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 6+ publications.
产品编号 ABIN2854880
发货至: 中国

Quick Overview for Nanog 抗体 (ABIN2854880)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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宿主

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克隆类型

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多克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 交叉反应

    Ceratotherium simum, 人, 小鼠

    产品特性

    Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
    Nanog antibody [N3C3]

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: HeLa nucleus

    Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Shen, Kang, Shakya, Tabaka, Jarboe, Regev, Tantin: "Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, (2018) (PubMed).

    Hsu, Chang, Hsu, Liu, Yeh, Wang, Liou, Ko, Chang, Chuang: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).

    Marinho, Chailangkarn, Muotri: "Systematic optimization of human pluripotent stem cells media using Design of Experiments." in: Scientific reports, Vol. 5, pp. 9834, (2016) (PubMed).

    Ju, Huang, Huang, Su: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).

    Singh, Chappell, Trost, Lin, Wang, Tang, Matlock, Weller, Wu, Zhao, Jin, Dalton: "Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells." in: Stem cell reports, Vol. 1, Issue 6, pp. 532-44, (2015) (PubMed).

    Liu, Bain: "Arsenic inhibits hedgehog signaling during P19 cell differentiation." in: Toxicology and applied pharmacology, Vol. 281, Issue 3, pp. 243-53, (2014) (PubMed).

  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    Nanog homeobox

    背景

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

    Cellular Localization: Nucleus

    分子量

    35 kDa

    基因ID

    79923

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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