Nanog 抗体
Quick Overview for Nanog 抗体 (ABIN2854880)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- Ceratotherium simum, 人, 小鼠
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产品特性
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Rabbit Polyclonal antibody to Nanog (Nanog homeobox)
Nanog antibody [N3C3] -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: HeLa nucleus
Validation: Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, (2018) (PubMed).
: "Suberoylanilide hydroxamic acid represses glioma stem-like cells." in: Journal of biomedical science, Vol. 23, Issue 1, pp. 81, (2017) (PubMed).
: "Systematic optimization of human pluripotent stem cells media using Design of Experiments." in: Scientific reports, Vol. 5, pp. 9834, (2016) (PubMed).
: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).
: "Cell-cycle control of developmentally regulated transcription factors accounts for heterogeneity in human pluripotent cells." in: Stem cell reports, Vol. 1, Issue 6, pp. 532-44, (2015) (PubMed).
: "Arsenic inhibits hedgehog signaling during P19 cell differentiation." in: Toxicology and applied pharmacology, Vol. 281, Issue 3, pp. 243-53, (2014) (PubMed).
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Enforcement of developmental lineage specificity by transcription factor Oct1." in: eLife, Vol. 6, (2018) (PubMed).
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- Nanog homeobox
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背景
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Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Cellular Localization: Nucleus -
分子量
- 35 kDa
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基因ID
- 79923
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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