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CDKN1B 抗体

This anti-CDKN1B antibody is a 兔 多克隆 antibody detecting CDKN1B in WB, IHC, IF, IHC (p), IP, ICC 和 IHC (fro). Suitable for 人. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN2854753
发货至: 中国

Quick Overview for CDKN1B 抗体 (ABIN2854753)

抗原

See all CDKN1B 抗体
CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

适用

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克隆类型

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多克隆

标记

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This CDKN1B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

质量等级

KO Validated
  • 特异性

    IP/MS validation was supported by references (PMID:30377371)

    交叉反应

    人, 小鼠, 大鼠

    产品特性

    Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
    p27 Kip1 antibody

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

    Validation: IP/MS, KO/KD, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.08 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Scully, Lahmy, Signaevskaia, Sasik, Medal, Kim, French, James, Wu, Lowy, Itkin-Ansari: "E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53." in: Cellular and molecular gastroenterology and hepatology, Vol. 6, Issue 2, pp. 181-198, (2018) (PubMed).

    Mlcochova, Caswell, Taylor, Towers, Gupta: "DNA damage induced by topoisomerase inhibitors activates SAMHD1 and blocks HIV-1 infection of macrophages." in: The EMBO journal, Vol. 37, Issue 1, pp. 50-62, (2018) (PubMed).

    Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).

  • 抗原

    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

    别名

    cyclin dependent kinase inhibitor 1B

    背景

    This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

    Cellular Localization: Nucleus , Cytoplasm

    分子量

    22 kDa

    基因ID

    1027

    UniProt

    P46527

    途径

    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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