我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

POLG 抗体 (C-Term)

Name: POLG 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2854742
Availability: OutOfStock

This anti-POLG antibody is a 兔多克隆 antibody detecting POLG in WB, ICC, IHC (p), IP 和 IF. Suitable for 人.

WB Image Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE antibody diluted at 1:1000 (POLG 抗体 (C-Term))

ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using DNA polymerase gamma, antibody at 1:200 dilution. (POLG 抗体 (C-Term))

IP Image Immunoprecipitation of DNA polymerase gamma protein from MCF-7 whole cell extracts using 5 μg of DNA polymerase gamma antibody, Western blot analysis was performed using DNA polymerase gamma antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (POLG 抗体 (C-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using DNA polymerase gamma, antibody(10 μg/ml). (POLG 抗体 (C-Term))

IHC

WB Image DNA polymerase gamma antibody detects POLG protein by Western blot analysis. (POLG 抗体 (C-Term))

5 images

产品编号 ABIN2854742

发货至: 中国

Quick Overview for POLG 抗体 (C-Term) (ABIN2854742)

抗原

See all POLG 抗体

POLG (Polymerase (DNA Directed), gamma (POLG))

适用

人

宿主

兔

克隆类型

多克隆

标记

This POLG antibody is un-conjugated

应用范围

Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

抗原表位
15

6

4

3

3

2

2

1

1

1

1

1

1
C-Term

交叉反应

人, 大鼠

产品特性

Rabbit Polyclonal antibody to DNA polymerase gamma (polymerase (DNA directed), gamma)
DNA polymerase gamma antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human DNA polymerase gamma. The exact sequence is proprietary.

亚型

IgG
anti-Polymerase (DNA Directed), gamma (POLG) (AA 1101-1239) antibody
POLG 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (AA 1041-1239) antibody
POLG 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) antibody
POLG 适用: 人 WB 宿主: 兔 Monoclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (AA 1120-1148), (C-Term) antibody
POLG 适用: 人, 小鼠 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (AA 1101-1239) antibody (AbBy Fluor® 488)
POLG 适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488

anti-Polymerase (DNA Directed), gamma (POLG) (AA 446-590) antibody
POLG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (AA 242-291) antibody
POLG 适用: 人, 小鼠, 大鼠, Cow, Bat, Hamster, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (N-Term) antibody
POLG 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (N-Term) antibody
POLG 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Saccharomyces cerevisiae, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), gamma (POLG) (AA 1101-1239) antibody (Biotin)
POLG 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal Biotin

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: Rat testis , MCF-7 , MDA-MB-231

限制

仅限研究用
状态

Liquid

浓度

0.67 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

POLG (Polymerase (DNA Directed), gamma (POLG))

别名

DNA polymerase gamma

背景

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Mitochondrion

分子量

140 kDa

基因ID

5428

UniProt

P54098