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beta Actin 抗体 (N-Term)

Name: beta Actin 抗体 (N-Term)
Brand: antibodies-online
SKU: ABIN2854709
Availability: OutOfStock

This anti-beta Actin antibody is a 兔多克隆 antibody detecting beta Actin in WB, IF, IHC (p) 和 ICC. Suitable for 人. This Primary Antibody has been cited in 4+ publications.

WB Image Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE Beta-actin antibody antibody diluted at 1:1000 (beta Actin 抗体 (N-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using beta Actin, antibody (Green) at 1:1000 dilution. (beta Actin 抗体 (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Actin, antibody at 1:500 dilution. (beta Actin 抗体 (N-Term))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE antibody diluted at 1:5000 (beta Actin 抗体 (N-Term))

WB Image Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody , diluted at 1:1000. (beta Actin 抗体 (N-Term))

IHC-P Image beta Actin antibody detects beta Actin protein at cytosol on mouse prostate by immunohistochemical analysis. (beta Actin 抗体 (N-Term))

IHC

6 images

(4 references)

产品编号 ABIN2854709

发货至: 中国

Quick Overview for beta Actin 抗体 (N-Term) (ABIN2854709)

抗原

See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))

适用

人

宿主

兔

克隆类型

多克隆

标记

This beta Actin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

质量等级

KO Validated

Discover our top product ACTB Primary Antibody

抗原表位
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N-Term

交叉反应

Cat, 犬, Drosophila melanogaster, 人, 小鼠

产品特性

Rabbit Polyclonal antibody to beta Actin (actin, beta)
beta Actin antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human beta Actin. The exact sequence is proprietary.

亚型

IgG
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated

anti-Actin, beta (ACTB) (Middle Region) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow, 绵羊, 斑马鱼, 马, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , Raji , 293T
Validation: KO/KD

限制

仅限研究用
状态

Liquid

浓度

1.16 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Yu, Luan, Liu, Su, Li: "MiR-296 Promotes Osteoblast Differentiation by Upregulating Cbfal." in: Pharmacology, Vol. 105, Issue 3-4, pp. 190-201, (2020) (PubMed).

Tao, Cheng, Yang: "Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway." in: Clinical interventions in aging, Vol. 15, pp. 373-385, (2020) (PubMed).

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

actin beta

背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Cellular Localization: Cytoplasm , cytoskeleton

分子量

42 kDa

基因ID

60

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction