我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

ACSL4 抗体 (C-Term)

Name: ACSL4 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2854678
Availability: OutOfStock

This anti-ACSL4 antibody is a 兔多克隆 antibody detecting ACSL4 in WB, IF, ICC, IHC (p) 和 IP. Suitable for 人. This Primary Antibody has been cited in 3+ publications.

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. (ACSL4 抗体 (C-Term))

ICC/IF Image FACL4 antibody [C3], C-term detects FACL4 protein at endoplasmic reticulum by immunofluorescent analysis. (ACSL4 抗体 (C-Term))

IP Image Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 μg of FACL4 antibody [C3], C-term, Western blot analysis was performed using FACL4 antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (ACSL4 抗体 (C-Term))

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FACL4, antibody (Green) at 1:500 dilution. (ACSL4 抗体 (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: Hela B: NT2D1 7. (ACSL4 抗体 (C-Term))

IHC-P Image FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on mouse lymph node by immunohistochemical analysis. (ACSL4 抗体 (C-Term))

IHC

IHC-P Image FACL4 antibody [C3], C-term detects FACL4 protein at cytosol on rat middle brain by immunohistochemical analysis. (ACSL4 抗体 (C-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using FACL4, antibody at 1:500 dilution. (ACSL4 抗体 (C-Term))

IHC

8 images

(3 references)

产品编号 ABIN2854678

发货至: 中国

Datasheet as PDF

Quick Overview for ACSL4 抗体 (C-Term) (ABIN2854678)

抗原

See all ACSL4 抗体

ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

适用

人

宿主

兔

克隆类型

多克隆

标记

This ACSL4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

抗原表位
10

8

6

6

6

3

2

2

2

1

1

1

1

1

1
C-Term

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to FACL4 (acyl-CoA synthetase long-chain family member 4)
FACL4 antibody [C3], C-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FACL4. The exact sequence is proprietary.

亚型

IgG
anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (AA 1-280) antibody
ACSL4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) antibody
ACSL4 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (C-Term) antibody
Verified ACSL4 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (AA 457-670) antibody
ACSL4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) antibody
ACSL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (N-Term) antibody
ACSL4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (AA 236-267) antibody
ACSL4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB04697 unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (N-Term) antibody
ACSL4 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 豚鼠, Pig, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (AA 244-274) antibody
ACSL4 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) (AA 236-267) antibody
ACSL4 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:1000-1:5000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

限制

仅限研究用
状态

Liquid

浓度

0.44 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Ndiaye, Liu, Hall, Minogue, Morgan, Waugh: "Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases." in: Bioscience reports, Vol. 40, Issue 4, (2020) (PubMed).

Garcia-Bermudez, Baudrier, Bayraktar, Shen, La, Guarecuco, Yucel, Fiore, Tavora, Freinkman, Chan, Lewis, Min, Inghirami, Sabatini, Birsoy: "Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death." in: Nature, Vol. 567, Issue 7746, pp. 118-122, (2019) (PubMed).

Radif, Ndiaye, Kalantzi, Jacobs, Hall, Minogue, Waugh: "The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells." in: Molecular and cellular biochemistry, Vol. 448, Issue 1-2, pp. 275-286, (2018) (PubMed).
抗原

ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))

别名

acyl-CoA synthetase long chain family member 4

背景

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

Cellular Localization: Mitochondrion outer membrane, Single-pass type III membrane protein , Peroxisome membrane, Single-pass type III membrane protein , Microsome membrane, Single-pass type III membrane protein , Endoplasmic reticulum membrane, Single-pass type III membrane pr

分子量

79 kDa

基因ID

2182

UniProt

O60488