PFKM 抗体 (N-Term)
Quick Overview for PFKM 抗体 (N-Term) (ABIN2854614)
抗原
See all PFKM 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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交叉反应
- 人, 小鼠, 大鼠
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产品特性
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Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)
PFK (muscle) antibody [N1N2-2], N-term -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the N-terminus region of human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.
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亚型
- IgG
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anti-phosphofructokinase, Muscle (PFKM) (AA 2-390) antibody
PFKM 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 501-780) antibodyPFKM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 166-334) antibodyPFKM 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 1-780) antibodyPFKM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 681-780) antibodyPFKM 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D1 unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 746-776), (C-Term) antibodyPFKM 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3908 unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 1-780) antibodyPFKM 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) antibodyPFKM 适用: 兔 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (AA 1-253) antibodyPFKM 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphofructokinase, Muscle (PFKM) (C-Term) antibodyPFKM 适用: 人, 小鼠, 大鼠, 斑马鱼, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: mouse brain , rat brain , HeLa
Validation: KO/KD
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.52 mg/mL
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缓冲液
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- PFKM (phosphofructokinase, Muscle (PFKM))
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别名
- phosphofructokinase, muscle
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背景
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The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL, MIM 171860), and platelet (PFKP, MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721], Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]
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分子量
- 85 kDa
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基因ID
- 5213
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UniProt
- P08237
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途径
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
抗原
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