Fluorescein 抗体
(2 references)
Quick Overview for Fluorescein 抗体 (ABIN2801850)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-1196
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Supplier
- Rockland
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原理
- Fluorescein Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Fluorescein conjugated goat IgG and Fluorescein conjugated KLH.
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产品特性
- Synonyms: goat anti-Fluorescein Antibody, goat anti-FITC antibody
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纯化方法
- Fluorescein Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
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Immunogen: Fluorescein conjugated Bovine Serum Albumin (BSA)
Immunogen Type: Native Protein
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anti-Fluorescein antibody
WB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS, IF 宿主: 小鼠 Monoclonal 8B9-C6-D3 unconjugated
anti-Fluorescein antibodyWB, ELISA, IHC, FACS 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Fluorescein Antibody has been assayed against 1.0 μg of Fluorescein conjugated Rabbit IgG in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Donkey) code #605-703-125 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 90 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).
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: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).
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- Fluorescein
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物质类
- Chemical
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背景
- Background: Anti-Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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