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GABARAP 抗体 (N-Term)

This anti-GABARAP antibody is a 兔 多克隆 antibody detecting GABARAP in WB, IHC 和 IF. Suitable for 人.
产品编号 ABIN2800631
发货至: 中国

Quick Overview for GABARAP 抗体 (N-Term) (ABIN2800631)

抗原

See all GABARAP 抗体
GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

适用

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宿主

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克隆类型

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多克隆

标记

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This GABARAP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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    AA 1-30, N-Term

    原理

    Rabbit Anti-Human GABARAP (N-term) Antibody

    免疫原

    This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.

    亚型

    Ig Fraction
  • 应用备注

    Immunofluorescence, Western Blot, Immunohistochemistry
    Recommended Dilutions
    IF: 1:10-50, WB: 1:1000, IHC: 1:10-50Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-13733 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Western blot analysis of GABARAP (arrow) using purified Pab .

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C (short-term), -20°C (long-term)
  • 抗原

    GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))

    别名

    GABARAP

    背景

    Target Description: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.

    Gene Symbol: GABARAP

    分子量

    13918 Da

    基因ID

    11337

    UniProt

    O95166

    途径

    Autophagy
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