GABARAP 抗体 (N-Term)
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北京 101111
Quick Overview for GABARAP 抗体 (N-Term) (ABIN2800631)
抗原
See all GABARAP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-30, N-Term
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原理
- Rabbit Anti-Human GABARAP (N-term) Antibody
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免疫原
- This GABARAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GABARAP.
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亚型
- Ig Fraction
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anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-100) antibody
GABARAP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-30) antibodyGABARAP 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11846 unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-30) antibodyGABARAP 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (AA 1-31), (N-Term) antibodyGABARAP 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibodyGABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (HRP)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal HRP
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (FITC)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal FITC
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (Biotin)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal Biotin
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (PE)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal PE
anti-GABA(A) Receptor-Associated Protein (GABARAP) (N-Term) antibody (APC)GABARAP 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal APC
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应用备注
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Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:10-50, WB: 1:1000, IHC: 1:10-50Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-13733 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.Western blot analysis of GABARAP (arrow) using purified Pab . -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C (short-term), -20°C (long-term)
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- GABARAP (GABA(A) Receptor-Associated Protein (GABARAP))
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别名
- GABARAP
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背景
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Target Description: Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. GABARAP is GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Gene Symbol: GABARAP
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分子量
- 13918 Da
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基因ID
- 11337
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UniProt
- O95166
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途径
- Autophagy
抗原
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