LIN28B 抗体 (N-Term)
Quick Overview for LIN28B 抗体 (N-Term) (ABIN2800623)
抗原
See all LIN28B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-30, N-Term
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原理
- Rabbit Anti-Human LIN28B (N-term) Antibody
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免疫原
- This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B.
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亚型
- Ig Fraction
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anti-Lin-28 Homolog B (LIN28B) (AA 1-30) antibody
LIN28B 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 95-128) antibodyLIN28B 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 166-250) antibodyLIN28B 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) antibodyLIN28B 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 218-250) antibodyLIN28B 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 95-128) antibodyLIN28B 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 200-250) antibodyLIN28B 适用: 人, 猴 IHC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (N-Term) antibodyLIN28B 适用: 人 WB, ELISA, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (Center) antibodyLIN28B 适用: 人 WB, FACS, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog B (LIN28B) (AA 218-250), (C-Term) antibodyLIN28B 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Recommended Dilutions
IF: 1:100, WB: 1:1000, IHC: 1:10-50, FC: 1:10-50Fluorescent confocal image of SY5Y cells stained with LIN28B (N-term) antibody. SY5Y cells were fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-12692 LIN28B (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).Western blot analysis of LIN28B Antibody (N-term) . FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.0 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C (short-term), -20°C (long-term)
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- LIN28B (Lin-28 Homolog B (LIN28B))
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别名
- LIN28B
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背景
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Target Description: Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.
Gene Symbol: LIN28B
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分子量
- 27084 Da
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基因ID
- 389421
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UniProt
- Q6ZN17
抗原
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