HDAC9 抗体 (C-Term)
Quick Overview for HDAC9 抗体 (C-Term) (ABIN2800619)
抗原
See all HDAC9 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 503-533, C-Term
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原理
- Rabbit Anti-Human HDAC9 (C-term) Antibody
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免疫原
- This HDAC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-533 amino acids from the C-terminal region of human HDAC9.
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亚型
- Ig Fraction
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应用备注
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Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Both anti-HDAC9 N-term (102-10933) and C-term (102-10934) Pab were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both Pab specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone. Bottom figure shows that both Pab can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated. BC = breast carcinoma, HC = hepatocarcinoma. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.000 mg/mL
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C (short-term), -20°C (long-term)
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- HDAC9 (Histone Deacetylase 9 (HDAC9))
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别名
- HDAC9
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背景
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Target Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Gene Symbol: HDAC9
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分子量
- 111297 Da
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基因ID
- 9734
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UniProt
- Q9UKV0
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
抗原
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