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OCT4 抗体 (N-Term)

This 兔多克隆 antibody specifically detects OCT4 in WB. It exhibits reactivity toward 人, Pig, 犬, 兔和山羊. It has been mentioned in 3+ publications

2 images

(3 references)

产品编号 ABIN2792671

发货至: 中国

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Quick Overview for OCT4 抗体 (N-Term) (ABIN2792671)

抗原

See all OCT4 (POU5F1) 抗体

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

人, Pig, 犬, 兔, 山羊

宿主

兔

克隆类型

多克隆

标记

This OCT4 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product POU5F1 Primary Antibody

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N-Term

序列

PCTVTPGAVK LEKEKLEQNP EESQDIKALQ KELEQFAKLL KQKRITLGYT

交叉反应

牛, 犬, 人, 猪, 兔

预测反应

Dog: 91%, Goat: 100%, Human: 100%, Pig: 84%, Rabbit: 92%

产品特性

This is a rabbit polyclonal antibody against POU5F1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human POU5F1
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
KO Validated POU5F1 适用: 人 WB, ICC, IP, IF, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibody
POU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibody
POU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibody
POU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-POU Class 5 Homeobox 1 (POU5F1) antibody
POU5F1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB1425 unconjugated Recombinant Antibody

应用备注

WB Suggested Anti-POU5F1 Antibody Titration: 1.25 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 360 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Li, Moretto-Zita, Soncin, Wakeland, Wolfe, Leon-Garcia, Pandian, Pizzo, Cui, Nazor, Loring, Crum, Laurent, Parast: "BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ?Np63+ cytotrophoblast stem cell state." in: Development (Cambridge, England), Vol. 140, Issue 19, pp. 3965-76, (2013) (PubMed).

Kaeffer, Legrand, Moyon, Frondas-Chauty, Billard, Guzman-Quevedo, Darmaun, Rozé: "Non-invasive exploration of neonatal gastric epithelium by using exfoliated epithelial cells." in: PLoS ONE, Vol. 6, Issue 10, pp. e25562, (2011) (PubMed).

Suo, Han, Wang, Zhang, Zhao, Zhao, Dai: "Oct4 pseudogenes are transcribed in cancers." in: Biochemical and biophysical research communications, Vol. 337, Issue 4, pp. 1047-51, (2005) (PubMed).
抗原

OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

别名

OCT3/4

背景

POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. It determines paracrine growth factor signaling from stem cells to the trophectoderm
Alias Symbols: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4
Protein Interaction Partner: ETS2, CETN2, XPC, RAD23B, UBC, ITCH, FOXD3, WWP2, PSMD10, NCL, PURA, PPIA, POU5F1, YBX1, KRT19, KRT14, KRT10, KRT9, KRT2, KRT1, HNRNPK, HMGB1, HIST1H1T, GAPDH, FKBP7, EHMT2, AGR2, TUBB4A, EIF5B, TUBA1A, NR2F2, TRA2B, SRSF3, RPS25, RPS23, RPS19, RPL31, RPL
Protein Size: 360

分子量

39 kDa

基因ID

5460

NCBI登录号

NM_002701, NP_002692

UniProt

Q01860

途径

Stem Cell Maintenance