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Plakophilin 2 抗体 (N-Term)

Name: Plakophilin 2 抗体 (N-Term)
SKU: ABIN2786784
Availability: OutOfStock
Rating: 5 (1 reviews)

PKP2 适用: 人, 大鼠, 小鼠, Cow, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Plakophilin 2 (PKP2) (N-Term) antibody (ABIN2786784)

WB Suggested Anti-PKP2 Antibody Titration: 0.

3 images

(1 validation)

产品编号 ABIN2786784

发货至: 中国

Datasheet as PDF

抗原 See all Plakophilin 2 (PKP2) 抗体

Plakophilin 2 (PKP2)
抗原表位
14

8

7

5

2

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
50

19

19

4

2

2

2

1

1

1

1

1
人, 大鼠, 小鼠, Cow, 犬, 马
宿主
43

4

2

1
兔
Compatible Secondaries
克隆类型
46

4
多克隆
标记
28

4

4

3

2

2

1

1

1

1

1

1

1
This Plakophilin 2 antibody is un-conjugated
应用范围
36

23

22

6

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

HPLRRLEISP DSSPERAHYT HSDYQYSQRS QAGHTLHHQE SRRAALLVPP

预测反应

Cow: 77%, Dog: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Rat: 77%

产品特性

This is a rabbit polyclonal antibody against PKP2. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human PKP2

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PKP2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

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anti-Plakophilin 2 (PKP2) (N-Term) antibody
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Verified PKP2 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Plakophilin 2 (PKP2) (AA 571-849) antibody
PKP2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Plakophilin 2 (PKP2) antibody
PKP2 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 837 AA

限制

仅限研究用
生效 #103654 (Western Blotting)

by

Gencardio (IDIBGI)

No.

#103654

日期

2019.03.13

抗原

PKP2

Lot Number

QC27639-43357

Method validated

Western Blotting

Positive Control

HL-1 cell extracts (from AT-1 mouse atrial cardiomyocyte tumor lineage)

Extracts from HEK293 cells transfected with human PKP2-FLAG expressing plasmid

Extract of mouse heart tissue (endogenous PKP2 expected molecular weight 88KDa)

Extract of human heart tissue (endogenous PKP2 expected molecular weight 91KDa)

Negative Control

Extracts from untransfected HEK293 cells

Notes

ABIN2786784 specifically recognizes PKP2 in cell extracts from HEK-293 overexpressing human PKP2 protein and endogenous PKP2 in human heart tissue but not endogenous mouse PKP2 neither in HL-1 cells nor mouse tissue.

Validation Images

Western blot of lysates from HEK293 cells transfected with FLAG-tagged PKP2 (1), non-transfected HEK293 cells (2), non-transfected HL1 cells (3) HEK293 cells transfected with ABIN2786784 or an anti-FLAG tag antibody. In addition, lysates from human (4) and mouse heart tissue (5, 6) were incubated with ABIN2786784.
Full Methods
Primary Antibody

ABIN2786784

Secondary Antibody

mouse anti-FLAG-tag antibody (Sigma, monoclonal anti-flag, F316, lot SLBT7654)

Full Protocol

Grow HL-1 cells in Claycomb medium (Sigma, 51800C, lot SLBX8700) supplemented with 10% FBS (Gibco, 10270-106, lot 42G5178K), 1% Penicillin-Streptomycin (Sigma, P4333, lot 048M4874V) and 1% Glutamax (Gibco, 35050-061, 2037045), at 37°C and 5% CO₂ in 2ml on a 6 well plate to 95% confluency.
Grow HEK293 cells in DMEM/High Modified medium (GE Life Science, SH30285.01, lot AC14565263) supplemented with 10% FBS, 1% Penicillin-Streptomycin and 1% Glutamax (Gibco, 35050-061, 2037045), at 37°C and 5% CO₂ in 2ml on a 6 well plate to 95% confluency.
Transfect HEK293 cells with PKP2a plasmid (p915 PKP2a-pFLAG-cmv5a; provided by Dr. Green KJ) using Lipofectamin 2000 (Invitrogen, 11668-019, lot 2022713) following the manufacturer´s instructions.
Lyse approximately 1.2x10⁶ cells per well in 200µl 1% SDS (Sigma, 75746, lot STBG4187V).
Homogenate whole heart of transgenic and wt heart mouse tissue with M-Per reagent (Thermo, 78501, lot NC168900) and mechanical disgregation using a Dounce homogenizer.
Incubate for the lysates from 15min at 95°C.
Vortex for 15min.
Determine total protein content of the lysates using Pierce BCA Protein Assay Kit (Thermo Scientific, 23227, lot TC263613).
Denature 15µg (HEK293 cell lysates), 106µg (HL-1 cell lysates), and 100µg (mouse heart tissue lysates) of total protein for 5min at 95°C in 45µl using 6x Laemmli SDS sample buffer and subsequently separate them on a 8% acrylamide gel in an electrophoresis chamber for 30min at 80V and 1h at 120V.
Transfer proteins onto Amersham Hybond P 0.45 PVDF blotting membrane (GE Life Science, 10600023, lot A16954274) for 2h at 80V.
Block the membrane with 5% NFM in PBST for 1h at RT.
Incubation with primary
rabbit anti-Plakophilin 2 (PKP2) (N-Term) antibody (antibodies-online, ABIN2786784, lot QC27639-43357) diluted 1:800 in 5% NFM in PBS ON at 4°C or
mouse anti-FLAG-tag antibody (Sigma, monoclonal anti-flag, F316, lot SLBT7654) diluted 1:x in 5% NFM in PBS ON at 4°C.
Wash membrane 2x for 5’ with PBST and 2x for 5min with PBS.
Block the membrane with 5% NFM in PBST for 1h at RT.
Incubation with secondary anti-rabbit (Jackson ImmunoResearch, 111-035-003, lot # 135002) diluted 1:10000 in 5% NFM in PBS for 1h at RT.
Wash membrane 2x for 5min with PBST and 2x for 5min with PBS.
Reveal protein bands using Perce ECL Western (Thermo Scientific, 32106, TE265714) with Amersham Hyperfilm ECL (GE Healthcare Limited, 28906835, 65901).

Experimental Notes

HEK293 cells with no endogenous PKP2 expression have been transfected with human FLAG-tagged PKP2 as a positive control. HL-1 cells express endogenous mouse PKP2.
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

Plakophilin 2 (PKP2)

别名

PKP2 (PKP2 产品)

别名

ARVD9 antibody, 1200008D14Rik antibody, 1200012P04Rik antibody, AA516617 antibody, Pkp2l antibody, plakophilin 2 antibody, PKP2 antibody, Pkp2 antibody

背景

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the
Alias Symbols: ARVD9
Protein Interaction Partner: NDEL1, MTUS2, CEP250, DROSHA, BMI1, EED, RNF2, YWHAB, CLK2, YWHAE, WHSC1, UBC, SMAD9, YWHAQ, CUL3, MARK3, POLR3A, YWHAG, PKP2, JUP, KRT18, KRT5, DSG2, DSG1, DSP, DSC2, DSC1, CTNNB1, GTF2B, SFN,
Protein Size: 837

分子量

93 kDa

基因ID

5318

NCBI登录号

NM_001005242, NP_001005242

UniProt

A0AV37

途径

Cell-Cell Junction Organization, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection