电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

HYAL1 抗体 (N-Term)

HYAL1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2784343
发货至: 中国
  • 抗原 See all HYAL1 抗体
    HYAL1 (Hyaluronidase-1 (HYAL1))
    抗原表位
    • 15
    • 10
    • 9
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    适用
    • 56
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔
    宿主
    • 54
    • 6
    • 1
    克隆类型
    • 58
    • 3
    多克隆
    标记
    • 32
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This HYAL1 antibody is un-conjugated
    应用范围
    • 42
    • 30
    • 22
    • 13
    • 13
    • 10
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    序列
    TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
    预测反应
    Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
    产品特性
    This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
    纯化方法
    Affinity Purified
    免疫原
    The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
    Top Product
    Discover our top product HYAL1 Primary Antibody
  • 应用备注
    Optimal working dilutions should be determined experimentally by the investigator.
    说明

    Antigen size: 435 AA

    限制
    仅限研究用
  • 生效 #100034 (Immunohistochemistry)
    '独立验证'标志
    by
    University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics
    No.
    #100034
    日期
    2016.08.16
    抗原
    HYAL1 antibody - N-terminal region
    Lot Number
    QC23783
    Method validated
    Immunohistochemistry
    Positive Control
    Testes of Hyal1 +/+ mouse
    Negative Control
    Testes of Hyal1 -/- mouse
    Notes
    ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2784343
    Secondary Antibody
    Goat-anti-rabbit AF568
    Full Protocol
    • Deparafinize and rehydrate:
      • xylene (I & II) – 2x 5min
      • 100% Ethanol (I & II) – 2x 5min
      • 95% Ethanol (I & II) – 2x 3min
      • 70% Ethanol (I) – 3min
      • 50% Ethanol (I) – 3min
      • ddH2O – 3min
    • Antigen retrieval:
      • Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
      • Incubate at 95°C for 20min.
      • Cool down at RT for 20min.
      • Wash twice with PBS for 5min.
    • Immunostaining:
      • Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
      • Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
      • Incubate at 4°C overnight.
      • Wash slides three times with TBST 5min.
      • Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
      • Incubate at RT for 1h.
      • Wash slides in TBST - 1x 5min at RT.
      • Wash three times with H2O for 5min.
      • Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
      • Wash twice with H2O for 5min.
      • Mounted with ProLong Gold (ThermoFisher, P36931) solution.
    Experimental Notes
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • 抗原
    HYAL1 (Hyaluronidase-1 (HYAL1))
    别名
    HYAL1 (HYAL1 产品)
    别名
    HYAL-1 antibody, LUCA1 antibody, NAT6 antibody, Hya1 antibody, Hyal-1 antibody, hyaluronoglucosaminidase 1 antibody, HYAL1 antibody, Hyal1 antibody
    背景
    HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
    Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
    Protein Interaction Partner: UBC, COL2A1,
    Protein Size: 435
    分子量
    45 kDa
    基因ID
    3373
    NCBI登录号
    NM_153282, NP_695014
    UniProt
    Q12794
    途径
    Glycosaminoglycan Metabolic Process
You are here:
客服