HYAL1 抗体 (N-Term)
(1 validation)Quick Overview for HYAL1 抗体 (N-Term) (ABIN2784343)
抗原
See all HYAL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
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预测反应
- Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
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产品特性
- This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
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anti-Hyaluronidase-1 (HYAL1) (N-Term) antibody
HYAL1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) antibodyHYAL1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (Internal Region) antibodyVerified HYAL1 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 32-47), (N-Term) antibodyHYAL1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 136-435) antibodyHYAL1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 1-253) antibodyHYAL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 271-400) antibodyHYAL1 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 136-435) antibodyHYAL1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 60-159) antibodyHYAL1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Hyaluronidase-1 (HYAL1) (AA 36-85) antibodyHYAL1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 435 AA
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限制
- 仅限研究用
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- by
- University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics
- No.
- #100034
- 日期
- 2016.08.16
- 抗原
- HYAL1 antibody - N-terminal region
- Lot Number
- QC23783
- Method validated
- Immunohistochemistry
- Positive Control
- Testes of Hyal1 +/+ mouse
- Negative Control
- Testes of Hyal1 -/- mouse
- Notes
- ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
- Primary Antibody
- ABIN2784343
- Secondary Antibody
- Goat-anti-rabbit AF568
- Full Protocol
- Deparafinize and rehydrate:
- xylene (I & II) – 2x 5min
- 100% Ethanol (I & II) – 2x 5min
- 95% Ethanol (I & II) – 2x 3min
- 70% Ethanol (I) – 3min
- 50% Ethanol (I) – 3min
- ddH2O – 3min
- Antigen retrieval:
- Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
- Incubate at 95°C for 20min.
- Cool down at RT for 20min.
- Wash twice with PBS for 5min.
- Immunostaining:
- Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
- Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
- Incubate at 4°C overnight.
- Wash slides three times with TBST 5min.
- Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
- Incubate at RT for 1h.
- Wash slides in TBST - 1x 5min at RT.
- Wash three times with H2O for 5min.
- Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
- Wash twice with H2O for 5min.
- Mounted with ProLong Gold (ThermoFisher, P36931) solution.
- Experimental Notes
生效 #100034 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as described in the protocol.]()
Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as described in the protocol.
Full Methods -
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- HYAL1 (Hyaluronidase-1 (HYAL1))
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别名
- HYAL1
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背景
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HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435 -
分子量
- 45 kDa
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基因ID
- 3373
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NCBI登录号
- NM_153282, NP_695014
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UniProt
- Q12794
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途径
- Glycosaminoglycan Metabolic Process
抗原
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