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OGT 抗体 (N-Term)

Name: OGT 抗体 (N-Term)
SKU: ABIN2783662
Availability: OutOfStock

OGT 适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, 豚鼠, 马, 兔, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated

Host: Rabbit Target Name: OGT Sample Type: 721_B Whole Cell lysates Antibody Dilution: 1. (OGT 抗体 (N-Term))

Rabbit Anti-OGT Antibody Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Cytoplasmic in processes of pinealocytes and endothelial cells in blood vessels Primary Antibody Concentration: 1:100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (OGT 抗体 (N-Term))

IHC

WB Suggested Anti-OGT Antibody Titration: 0. (OGT 抗体 (N-Term))

3 images

(1 reference)

产品编号 ABIN2783662

发货至: 中国

Datasheet as PDF

抗原 See all OGT 抗体

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
抗原表位
5

4

4

2

2

1

1

1

1

1

1

1
N-Term
适用
55

28

26

1

1

1

1

1

1

1

1

1

1

1
人, 小鼠, 大鼠, 犬, 斑马鱼, 豚鼠, 马, 兔, Cow
宿主
53

2

1
兔
Compatible Secondaries
克隆类型
42

14
多克隆
标记
31

3

3

3

3

3

3

3

3

1
This OGT antibody is un-conjugated
应用范围
32

16

15

11

10

7

5

4

2

2

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

序列

ASSVGNVADS TEPTKRMLSF QGLAELAHRE YQAGDFEAAE RHCMQLWRQE

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

产品特性

This is a rabbit polyclonal antibody against OGT. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human OGT

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Verified OGT 适用: 人, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 857-1036) antibody
OGT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IF, IHC (p), IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 606-1022) antibody
OGT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 8G7 unconjugated Recombinant Antibody

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠 WB, ChIP-seq, ChIP 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibody
OGT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibody
OGT 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 1046 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Taylor, Parker, Hazel, Soesanto, Fuller, Yazzie, McClain: "Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6050-7, (2008) (PubMed).
抗原

OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

别名

OGT (OGT 产品)

别名

BcDNA:GH04245 antibody, CG10392 antibody, Dmel\\CG10392 antibody, OGT antibody, Ogt antibody, P1201 antibody, SXC antibody, Sxc antibody, l(2)02637 antibody, l(2)NC130 antibody, sxc/Ogt antibody, fm81g08 antibody, ogt antibody, wu:fc12b01 antibody, wu:fm81g08 antibody, HRNT1 antibody, O-GLCNAC antibody, 1110038P24Rik antibody, 4831420N21Rik antibody, AI115525 antibody, Ogtl antibody, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog antibody, super sex combs antibody, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 antibody, O-linked N-acetylglucosamine (GlcNAc) transferase antibody, O-linked GlcNAc transferase antibody, o-linked GlcNAc transferase antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, ogt.L antibody, sxc antibody, ogt.1 antibody, OGT antibody, ogt antibody, GL50803_12081 antibody, GL50803_41701 antibody, THAPSDRAFT_264441 antibody, spyA antibody, Ogt antibody

背景

OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: FLJ23071, HRNT1, MGC22921, O-GLCNAC
Protein Interaction Partner: FBXW11, HUWE1, UBC, PSG1, PHC3, NUP62CL, TET2, SUMO1, NEDD8, WWOX, BAP1, P3H1, RPRD1B, PPME1, LAP3, DBNL, HSPBP1, GPN1, WDR4, ACTR2, TOM1L1, ZPR1, SURF2, LPP, AGFG1, ALAD, C14orf142, UBL4A, FOXO4, HCFC1, SMURF1, Hoxa1, Nfatc1, Nup62, RELA, PPP1CB, PPP1CA,
Protein Size: 1046

分子量

117 kDa

基因ID

8473

NCBI登录号

NM_181672, NP_858058

UniProt

O15294

途径

Regulation of Carbohydrate Metabolic Process