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RAD17 抗体 (C-Term)

Name: RAD17 抗体 (C-Term)
SKU: ABIN2779300
Availability: OutOfStock

This anti-RAD17 antibody is a 兔多克隆 antibody detecting RAD17 in WB. Suitable for 人, 小鼠, 大鼠, 犬, Cow 和 Pig. This Primary Antibody has been cited in 1 publication.

WB Suggested Anti-RAD17 Antibody Positive Control: Lane1: 25ug Hela lysate, Lane2: 25ug HEK293T lysate, Lane3: 25ug Xenopus laevis egg extract, Lane4: 25ug mouse embryonic stem cells lysate Primary Antibody Dilution : 1:500 Secondary Antibody : Anti-rabbit-HRP Secondry Antibody Dilution : 1:3000 Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS (RAD17 抗体 (C-Term))

WB Suggested Anti-RAD17 Antibody Titration: 1. (RAD17 抗体 (C-Term))

2 images

(1 reference)

产品编号 ABIN2779300

发货至: 中国

Datasheet as PDF

Quick Overview for RAD17 抗体 (C-Term) (ABIN2779300)

抗原

See all RAD17 抗体

RAD17

适用

人, 小鼠, 大鼠, 犬, Cow, Pig

宿主

兔

克隆类型

多克隆

标记

This RAD17 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product RAD17 Primary Antibody

抗原表位
20

15

9

7

7

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

序列

PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI IIEDYESDGT

预测反应

Cow: 83%, Dog: 75%, Human: 100%, Mouse: 75%, Pig: 83%, Rat: 75%

产品特性

This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17
anti-RAD17 (RAD17) (AA 1-584) antibody
RAD17 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) (AA 1-110) antibody
RAD17 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G12 unconjugated

anti-RAD17 (RAD17) (AA 391-670) antibody
RAD17 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) (AA 1-270) antibody
RAD17 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) (C-Term) antibody
RAD17 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) (C-Term) antibody
RAD17 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) (AA 621-670) antibody
RAD17 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) antibody
RAD17 适用: 人, 小鼠, 大鼠 WB, IF, IP, IHC (p) 宿主: 小鼠 Monoclonal 3-10E-010 unconjugated

anti-RAD17 (RAD17) (pSer646) antibody
RAD17 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-RAD17 (RAD17) antibody
RAD17 适用: 人, 小鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 584 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Tsao, Geisen, Abraham: "Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." in: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).
抗原

RAD17

别名

RAD17

背景

RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.
Alias Symbols: CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP
Protein Interaction Partner: USP20, UBC, PRMT6, CBX7, CBX6, RAD9A, CDH1, ATR, RFC1, H2AFX, FOXO3, ATM, CLSPN, ALK, RAD9B, POLE, HUS1, RAD1, NHP2L1, MCM7, RFC4, RFC5, RFC3, RFC2, POLE4, POLE3, POLE2, PRKDC,
Protein Size: 584

分子量

66 kDa

基因ID

5884

NCBI登录号

NM_133341, NP_579919