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Cyclin D1 抗体 (Middle Region)

Name: Cyclin D1 抗体 (Middle Region)
SKU: ABIN2779291
Availability: OutOfStock

This anti-Cyclin D1 antibody is a 兔多克隆 antibody detecting Cyclin D1 in WB. Suitable for 人, 大鼠, Cow, 兔, 犬, 斑马鱼, 马, Pig 和 Saccharomyces cerevisiae.

Lanes : Lane 1: 30ug PKO lysateLane 2: 30ug PKO + Doxor lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Goat anti rabbit-HRP Secondary Antibody Dilution : 1:5000 Gene Name : CCND1 Submitted by : Tobias Wagner, Institute for Biochemistry and Biophysics (Cyclin D1 抗体 (Middle Region))

Rabbit Anti-CCND1 Antibody Catalog Number: ARP30163_P050 Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm, Plasma membrane Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (Cyclin D1 抗体 (Middle Region))

IHC

WB Suggested Anti-CCND1 Antibody Titration: 0. (Cyclin D1 抗体 (Middle Region))

3 images

产品编号 ABIN2779291

发货至: 中国

Quick Overview for Cyclin D1 抗体 (Middle Region) (ABIN2779291)

抗原

See all Cyclin D1 (CCND1) 抗体

Cyclin D1 (CCND1)

适用

人, 大鼠, Cow, 兔, 犬, 斑马鱼, 马, Pig, Saccharomyces cerevisiae

宿主

兔

克隆类型

多克隆

标记

This Cyclin D1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product CCND1 Primary Antibody

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Middle Region

序列

ACQEQIEALL ESSLRQAQQN MDPKAAEEEE EEEEEVDLAC TPTDVRDVDI

预测反应

Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Pig: 91%, Rabbit: 83%, Rat: 85%, Yeast: 83%, Zebrafish: 79%

产品特性

This is a rabbit polyclonal antibody against CCND1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of human CCND1
anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 适用: 人, 小鼠, 大鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
KO Validated CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 167-295) antibody
CCND1 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
CCND1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 295 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

Cyclin D1 (CCND1)

别名

CCND1

背景

CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCL1, D11S287E, PRAD1, U21B31
Protein Interaction Partner: CDKN1B, CDKN1A, CDK4, RELA, FBXO31, UBC, FBXO4, CAMK1, TP73, RB1, CDK2, HSPA8, POLR1B, MCM10, ORC4, LPL, BRINP1, CDK6, RABEP1, CARD11, CUL1, RBL2, UBTF, CCND1, ATF2, CDKN2A, FBXW8, BTRC, PPP1CC, PPP1CB, PSMA6, ITLN1, MCM7, MCM6, MCM3, MCM2, ELAVL1, PCNA,
Protein Size: 295

分子量

34 kDa

基因ID

595

NCBI登录号

NM_053056, NP_444284

UniProt

P24385

途径

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling