GAPDH 抗体 (C-Term)
Quick Overview for GAPDH 抗体 (C-Term) (ABIN2778656)
抗原
See all GAPDH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- FNSNSHSSTF DAGAGIALND NFVKLISWYD NEYGYSNRVV DLMAYMASKE
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预测反应
- Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%
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产品特性
- This is a rabbit polyclonal antibody against Gapdh. It was validated on Western Blot.
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纯化方法
- Affinity Purified
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免疫原
- The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Gapdh
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
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说明
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Antigen size: 333 AA
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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别名
- Gapdh
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背景
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Gapdh has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Gapdh also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Alias Symbols: Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239
Protein Interaction Partner: Isg15, Kcnma1, Klhl40, Eed, Fbxo32, Lipc, Nanog, Siah1a, Ywhae,
Protein Size: 333 -
分子量
- 36 kDa
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基因ID
- 14433
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NCBI登录号
- NM_008084, NP_032110
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UniProt
- P16858
抗原
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