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SOX10 抗体 (Middle Region)

There is 1 publication for this product available. The 兔多克隆 anti-SOX10 antibody is suitable to detect SOX10 in samples from 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马和兔. It has been validated for WB 和 IHC.

A/ [IHC-PZ] (optimal processing) human optic nerve (short post mortem interval) fixed by immersion in zinc-based fixative (BD Pharmingen 552658), processed to minimize antigen loss (shortened protocol, reduced exposure to high temperature); embedded in paraffin wax; sectioned at 3 microns A2/ [IHC-PZ + FF] As above for initial fixation then post-fixed in 10% buffered formalin for 5 days B/ [IHC-P] human optic nerve and spinal cord fixed in 10% buffered formalin (relatively short post mortem interval and fixation duration); standard processing; embedded in paraffin wax; sectioned at 3 microns Controls Negative: omission of primary. (SOX10 抗体 (Middle Region))

IHC

WB Suggested Anti- (SOX10 抗体 (Middle Region))

Immunohistochemistry with Skin tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody (SOX10 抗体 (Middle Region))

IHC

Image no. 4 for anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (Middle Region) antibody (ABIN2777864) (SOX10 抗体 (Middle Region))

Host: Rabbit Target Name: SOX10 Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 0. (SOX10 抗体 (Middle Region))

SOX10 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000. (SOX10 抗体 (Middle Region))

Host: Rabbit Target Name: SOX10 Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 1ug/ml (SOX10 抗体 (Middle Region))

Immunohistochemistry with Kidney lysate tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody (SOX10 抗体 (Middle Region))

IHC

Host: Rabbit Target Name: SOX10 Sample Tissue: Jurkat cell lysatesLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 2. (SOX10 抗体 (Middle Region))

9 images

(1 reference)

产品编号 ABIN2777864

发货至: 中国

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Quick Overview for SOX10 抗体 (Middle Region) (ABIN2777864)

抗原

See all SOX10 抗体

SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

适用

人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔

宿主

兔

克隆类型

多克隆

标记

This SOX10 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product SOX10 Primary Antibody

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Middle Region

原理

SOX10 Antibody - middle region

序列

PGGEAEQGGT AAIQAHYKSA HLDHRHPGEG SPMSDGNPEH PSGQSHGPPT

预测反应

Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 86%

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the middle region of human SOX10
anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibody
SOX10 适用: 人, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal SOX10-991 unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 231-259) antibody
SOX10 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB21835 unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 147-252) antibody
SOX10 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2E7E8 unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 147-252) antibody
SOX10 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2E7B5 unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibody
SOX10 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal SOX10-992 unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibody
SOX10 适用: 人, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal rSOX10-1074 unconjugated Recombinant Antibody

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 115-269) antibody
SOX10 适用: 人, 小鼠 WB, IHC 宿主: 兔 Monoclonal SOX10-2311R unconjugated Recombinant Antibody

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 200-300) antibody
SOX10 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) antibody
SOX10 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Monoclonal 8E1 unconjugated Recombinant Antibody

anti-SRY (Sex Determining Region Y)-Box 10 (SOX10) (AA 416-466) antibody
SOX10 适用: 人, 小鼠 WB, IHC (fp), ICC, IP, IF (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

说明

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP33326-100UG

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

prevent freeze-thaw cycles

储存条件

4 °C,-20 °C

储存方法

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Wilson, Olm-Shipman, Acevedo, Palaniyandi, Hall, Kosa, Stumpff, Smith, Pitstick, Liao, Bjork, Czirok, Saadi: "SPECC1L deficiency results in increased adherens junction stability and reduced cranial neural crest cell delamination." in: Scientific reports, Vol. 6, pp. 17735, (2016) (PubMed).
抗原

SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

别名

SOX10

背景

Background Information: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. It acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease.
Gene Name: SRY (sex determining region Y)-box 10
Alternative Symbols: DOM, WS4, PCWH, WS2E, WS4C
Protein Name: Transcription factor SOX-10

分子量

50 kDa

基因ID

6663

NCBI登录号

NP_008872

UniProt

P56693

途径

Chromatin Binding