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Nanog 抗体 (Middle Region)

This anti-Nanog antibody is a 兔 多克隆 antibody detecting Nanog in WB. Suitable for 人, 小鼠, 大鼠, Cow, 犬, 绵羊, 山羊, 马, 兔 和 豚鼠. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN2777175
发货至: 中国

Quick Overview for Nanog 抗体 (Middle Region) (ABIN2777175)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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人, 小鼠, 大鼠, Cow, 犬, 绵羊, 山羊, 马, 兔, 豚鼠

宿主

  • 131
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克隆类型

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  • 31
多克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    Middle Region

    序列

    SYKQVKTWFQ NQRMKSKRWQ KNNWPKNSNG VTQKASAPTY PSLYSSYHQG

    预测反应

    Cow: 86%, Dog: 93%, Goat: 86%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 86%, Rat: 92%, Sheep: 86%

    产品特性

    This is a rabbit polyclonal antibody against NANOG. It was validated on Western Blot.

    纯化方法

    Affinity Purified

    免疫原

    The immunogen is a synthetic peptide directed towards the middle region of human NANOG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator.

    说明

    Antigen size: 305 AA

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freeze-thaw cycles.

    储存条件

    -20 °C

    储存方法

    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Kallas, Pook, Maimets, Zimmermann, Maimets: "Nocodazole treatment decreases expression of pluripotency markers Nanog and Oct4 in human embryonic stem cells." in: PLoS ONE, Vol. 6, Issue 4, pp. e19114, (2011) (PubMed).

    Yang, Atkinson, Vilella, Lloret, Armstrong, Mann, Lako: "Opposing putative roles for canonical and noncanonical NF?B signaling on the survival, proliferation, and differentiation potential of human embryonic stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 11, pp. 1970-80, (2010) (PubMed).

    Neganova, Zhang, Atkinson, Lako: "Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells." in: Oncogene, Vol. 28, Issue 1, pp. 20-30, (2009) (PubMed).

    Maimets, Neganova, Armstrong, Lako: "Activation of p53 by nutlin leads to rapid differentiation of human embryonic stem cells." in: Oncogene, Vol. 27, Issue 40, pp. 5277-87, (2008) (PubMed).

  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    NANOG

    背景

    NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
    Alias Symbols: -
    Protein Interaction Partner: DOT1L, UBC, ZNF281, RHOXF2, SALL4, MED12,
    Protein Size: 305

    分子量

    34 kDa

    基因ID

    79923

    NCBI登录号

    NM_024865, NP_079141

    UniProt

    Q6NSW7

    途径

    Stem Cell Maintenance
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