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alpha Actinin 4 抗体 (N-Term)

Name: alpha Actinin 4 抗体 (N-Term)
SKU: ABIN2775288
Availability: OutOfStock

This anti-alpha Actinin 4 antibody is a 兔多克隆 antibody detecting alpha Actinin 4 in WB 和 IHC. Suitable for 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼, 马和兔. This Primary Antibody has been cited in 2+ publications.

WB Suggested Anti-ACTN4 Antibody Titration: 0. (alpha Actinin 4 抗体 (N-Term))

Lanes: Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate Primary Antibody Dilution: 1: 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: ACTN4 Submitted by: Johannes W. (alpha Actinin 4 抗体 (N-Term))

Sample Type : ACTNX-GFP transfected COS-7 cells Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit-Alexa-Fluor 568 Secondary Antibody Dilution : 1:100 Color/Signal Descriptions : Green: GFP Red: ACTN4 Gene Name : ACTN4 Submitted by : Johannes W. (alpha Actinin 4 抗体 (N-Term))

IHC

4 images

(2 references)

产品编号 ABIN2775288

发货至: 中国

Quick Overview for alpha Actinin 4 抗体 (N-Term) (ABIN2775288)

抗原

See all alpha Actinin 4 (ACTN4) 抗体

alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

适用

人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼, 马, 兔

宿主

兔

克隆类型

多克隆

标记

This alpha Actinin 4 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product ACTN4 Primary Antibody

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N-Term

序列

LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM LDAEDIVNTA

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

产品特性

This is a rabbit polyclonal antibody against ACTN4. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Protein A purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN4
anti-Actinin, alpha 4 (ACTN4) (AA 592-701) antibody
ACTN4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated

anti-Actinin, alpha 4 (ACTN4) (AA 1-269) antibody
ACTN4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody
ACTN4 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) antibody
ACTN4 适用: 人 WB, IP, IF 宿主: 兔 Monoclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) (AA 160-440) antibody
ACTN4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) antibody
ACTN4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) (AA 1-911) antibody
ACTN4 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) (AA 561-661) antibody
ACTN4 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actinin, alpha 4 (ACTN4) (AA 785-813), (C-Term) antibody
ACTN4 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15705 unconjugated

anti-Actinin, alpha 4 (ACTN4) (C-Term) antibody
ACTN4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 911 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Wei, Gao, Du, Su, Xu: "Angiogenin enhances cell migration by regulating stress fiber assembly and focal adhesion dynamics." in: PLoS ONE, Vol. 6, Issue 12, pp. e28797, (2011) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).
抗原

alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

别名

ACTN4

背景

Alpha actinins belong to the spectrin superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
Alias Symbols: DKFZp686K23158, FSGS, FSGS1, ACTININ-4
Protein Interaction Partner: MYOZ2, HUWE1, UBC, BMP7, ACTN3, SUMO2, SUMO3, GNB2, SUMO1, NEDD8, MDM2, HGS, SF3A3, VCP, CDKN2A, ACTN1, PDLIM1, UBD, IGSF8, ICAM1, CD81, SVIL, Fcho2, POT1, VCAM1, TXN, RPS27, MAPK7, KPNA2, ITGA4, FN1, ESR1, CDH1, TRAF3IP1, FMNL1, NOS3, ACTN2, UBASH3B, SHC
Protein Size: 911

分子量

100 kDa

基因ID

81

NCBI登录号

NM_004924, NP_004915

UniProt

O43707

途径

Proton Transport