CHFR 抗体 (N-Term)
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- 抗原 See all CHFR 抗体
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
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抗原表位
- N-Term
- 适用
- 人, 小鼠, 大鼠, Cow, 兔, 斑马鱼, 犬, 马, 豚鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHFR antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 序列
- REWTIGRRRG CDLSFPSNKL VSGDHCRIVV DEKSGQVTLE DTSTSGTVIN
- 预测反应
- Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%
- 产品特性
- This is a rabbit polyclonal antibody against CHFR. It was validated on Western Blot using a cell lysate as a positive control.
- 纯化方法
- Affinity Purified
- 免疫原
- The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR
- Top Product
- Discover our top product CHFR Primary Antibody
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anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-652) antibody
CHFR 适用: 人 ELISA, WB, IHC (p), RNAi 宿主: 小鼠 Monoclonal 1H3-A12 unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 141-422) antibodyCHFR 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 136-194) antibodyCHFR 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) antibodyCHFR 适用: 人, 小鼠, 大鼠 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 36-85) antibodyCHFR 适用: 人, 小鼠, 大鼠, 兔, 斑马鱼, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (C-Term) antibodyCHFR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) antibodyCHFR 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (C-Term) antibodyVerified CHFR 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) (AA 1-290) antibodyCHFR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 说明
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Antigen size: 623 AA
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- 抗原
- CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))
- 别名
- CHFR (CHFR 产品)
- 别名
- RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody
- 背景
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CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
Alias Symbols: FLJ10796, FLJ33629, RNF116, RNF196
Protein Interaction Partner: USP7, VIM, UBE2D2, AURKA, HLTF, RANBP2, PLK1, PCNA, MCM2, HDAC2, HDAC1, CDK5, CDC20, PARP1, SIN3A, CHFR, UBC13, SUMO1, UBE2I, LRP1, UBE2V1, UBE2N, UBE2D1, TRIM9, RNF125, CBLC, RNF7, RELA, RNF8, HIST2H2BE, HIST2H2AC, UBC, SMARCD1, SMARCB1, SMARCA4, KIF22,
Protein Size: 623 - 分子量
- 69 kDa
- 基因ID
- 55743
- NCBI登录号
- NM_018223, NP_060693
- UniProt
- Q96EP1
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