CD82 抗体
(6 references)Quick Overview for CD82 抗体 (ABIN2749189)
抗原
See all CD82 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu CD82 Purified Low Endotoxin
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特异性
- The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
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交叉反应 （详细）
- Human, Other not tested
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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内毒素水平
- Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
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免疫原
- C91/PL (human HTLV-1+ T cell line)
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亚型
- IgG2a
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anti-CD82 (CD82) antibody
CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 unconjugated
anti-CD82 (CD82) antibodyCD82 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-CD82 (CD82) (AA 239-267), (C-Term) antibodyCD82 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB02073-02074 unconjugated
anti-CD82 (CD82) antibodyCD82 适用: 人 IHC, FACS 宿主: 小鼠 Monoclonal B-L2 unconjugated
anti-CD82 (CD82) (AA 98-267) antibodyCD82 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD82 (CD82) (AA 111-228) antibodyCD82 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD82 (CD82) (AA 1-267) antibodyCD82 适用: 人 ELISA, PLA 宿主: 小鼠 Monoclonal 4F2 unconjugated
anti-CD82 (CD82) (C-Term) antibodyCD82 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD82 (CD82) antibody (FITC)CD82 适用: 人 FACS 宿主: 小鼠 Monoclonal C33 FITC
anti-CD82 (CD82) antibody (Biotin)CD82 适用: 人 WB, FACS, IHC (p), IP, ICC 宿主: 小鼠 Monoclonal C33 Biotin
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应用备注
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Functional application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: Recommended dilution: 1-4 μg/mL -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).
: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, (1996) (PubMed).
: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).
: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).
: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).
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: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).
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- CD82
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别名
- CD82
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背景
- CD82 Molecule,CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.,R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27
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基因ID
- 3732
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UniProt
- P27701
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途径
- p53 Pathway
抗原
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