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Ki-67 抗体

This anti-Ki-67 antibody is a 小鼠 多克隆 antibody detecting Ki-67 in IHC, WB, FACS 和 ICC. Suitable for 人 和 Cow. This Primary Antibody has been cited in 3+ publications.
产品编号 ABIN2749188
发货至: 中国

Quick Overview for Ki-67 抗体 (ABIN2749188)

抗原

See all Ki-67 (MKI67) 抗体
Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

适用

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人, Cow

宿主

  • 100
  • 97
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小鼠

克隆类型

  • 119
  • 85
多克隆

标记

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  • 1
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This Ki-67 antibody is un-conjugated

应用范围

  • 131
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Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • 原理

    Anti-Ki-67 Purified

    特异性

    The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

    交叉反应 (详细)

    Human, Bovine

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    Nuclei of the Hodgkin lymphoma cell line L428

    亚型

    IgG1
  • 应用备注

    Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
    Immunocytochemistry: Paraformaldehyde fixation, recommended antibody concentration 1 μg/mL.

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Schlüter, Duchrow, Wohlenberg, Becker, Key, Flad, Gerdes: "The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins." in: The Journal of cell biology, Vol. 123, Issue 3, pp. 513-22, (1993) (PubMed).

    Gerdes, Lemke, Baisch, Wacker, Schwab, Stein: "Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 4, pp. 1710-5, (1984) (PubMed).

    Gerdes, Schwab, Lemke, Stein: "Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation." in: International journal of cancer. Journal international du cancer, Vol. 31, Issue 1, pp. 13-20, (1983) (PubMed).

  • 抗原

    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

    别名

    Ki-67

    背景

    Marker of proliferation Ki-67,Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.,KIA, MIB, Marker of proliferation Ki67, MKI67

    基因ID

    4288

    UniProt

    P46013

    途径

    Glycosaminoglycan Metabolic Process
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