This kappa Light Chain antibody is conjugated to Biotin
应用范围
Flow Cytometry (FACS)
原理
Anti-Hu Ig Kappa Light Chain Biotin
特异性
The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
交叉反应 (详细)
Human
纯化方法
Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
Flow cytometry: Recommended dilution: 1-12 μg/mL. Extracellular and intracellular staining.
限制
仅限研究用
浓度
1 mg/mL
缓冲液
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 2-8°C. Do not freeze.
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Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin kappa, Igk