(8 references)-
- 抗原 See all CD28 抗体
- CD28
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD28 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
- 交叉反应 （详细）
- Rat
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Mouse A20J B lymphoma cells transfected with rat CD28
- 克隆位点
- JJ319
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD28 Primary Antibody
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anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (AA 19-152) antibodyCD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibody (FITC)CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC
anti-CD28 (CD28) antibodyCD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated
anti-CD28 (CD28) antibodyCD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated
anti-CD28 (CD28) (C-Term) antibodyCD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-CD28 (CD28) (Internal Region) antibodyVerified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- Flow cytometry: Recommended dilution: 1-5 μg/mL.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).
: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, (2004) (PubMed).
: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, (2003) (PubMed).
: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, (2002) (PubMed).
: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, (2002) (PubMed).
: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, (2001) (PubMed).
: "Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation." in: Transplantation, Vol. 67, Issue 3, pp. 392-8, (1999) (PubMed).
: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).
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: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).
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- 抗原
- CD28
- 别名
- CD28 (CD28 产品)
- 背景
- CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,Tp44
- 基因ID
- 25660
- UniProt
- P31042
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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