MC4R 抗体 (AA 10-60) (FITC)
Quick Overview for MC4R 抗体 (AA 10-60) (FITC) (ABIN2746857)
抗原
See all MC4R 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-60
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特异性
- The antibody labels a strong band of MC4R at 40 kDa in ABIN1741693 samples and in other cancer cell lines.
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交叉反应
- 小鼠, 大鼠
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产品特性
- MC4R selective antibodies were generated against a peptide taken from the N-terminal region. The MC4R-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies are then stabilized in antibody stabilization buffer for long-term storage. The anti- MC4Rselective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides MC4R Western blot positive control samples in ready-to-use SDS-PAGE sample buffer. Limited quantities of antigenic blocking peptide for MC4R antibody is also available, please inquire before ordering.
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纯化方法
- Affinity Purified
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免疫原
- Synthetic peptide taken within amino acid region 10-60 on human MC4R protein.
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亚型
- IgG
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anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody
MC4R 适用: 人, 大鼠, 小鼠 ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (N-Term) antibodyMC4R 适用: 人, 猴, 马 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 6-20), (Extracellular) antibodyMC4R 适用: 人 WB, IHC, IF, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 25-57) antibodyMC4R 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB52063 unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 21-33) antibodyMC4R 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (Cytoplasmic Domain) antibodyMC4R 适用: 人, 绵羊, 兔, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠, 绵羊, 兔, Cow, 猴, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody (AbBy Fluor® 488)MC4R 适用: 人, 大鼠, 小鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Melanocortin 4 Receptor (MC4R) (N-Term) antibodyRecent MC4R 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.55-0.75 μg/μL
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注意事项
- Working solutions of antibodies in buffer should be filtered through 0.45 μm filter after every use for long-term storage.
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储存条件
- -20 °C
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储存方法
- For long-term storage of keep at -20 °C. We don't recommend storage of very dilute antibody solutions unless they are prepared in specially formulated multi use antibody dilution buffer.
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- MC4R (Melanocortin 4 Receptor (MC4R))
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别名
- MC4 Receptor
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背景
- Melanocortin receptors are members of the rhodopsin family of 7-trans membrane G protein-coupled receptors that are involved in multiple signal transduction pathways including the cAMP and MAPK signaling pathways. These receptors are activated by members of the Melanocortin family: alpha-, beta- and Gammamelanocyte stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). The Melanocortins are involved in a wide range of physiological functions, including pigmentation, energy homeostasis, inflammation, immunomodulation, steroidogenesis and temperature control. There are five melanocortin receptor subtypes each of which has a different pattern of tissue expression with the relative potency of different melanocortin peptides. The melanocortin signaling system consists of two endogenous antagonists that bind specifically to the receptor but instead of activating they have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP). Melanocortin-4 receptor (MC4R) transcripts are expressed primarily in the brain. Genetic studies of mice and humans established a critical role of MC4R in appetite regulation. MC4R has been implicated in weight regulation and defects in this gene are a cause of autosomal dominant obesity. Activation of MC4R results in the inhibition of c-Jun N-terminal kinase (JNK) activity and promotes insulin signaling. MC4R-null mice display maturity onset obesity characterized by hyperphagia, increased adiposity, hyperinsulinaemia and hyperleptinaemia, suggesting that obesity-linked gene, such as, MC4R is a potential candidate target for the treatment of obesity. Heterozygous mutations in MC4R account for 1-6 % of severe cases of human obesity. All known mammalian MC4Rs are 332 amino acids in length with an estimated molecular weight of 40 kDa. The protein is heavily glycosylated and appears at multiple positions on SDS-PAGE. The gene for MC4R is present on chromosome 18q22.
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NCBI登录号
- NP_005903
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UniProt
- P32245
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途径
- Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
抗原
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