MC4R 抗体 (Extracellular)
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Quick Overview for MC4R 抗体 (Extracellular) (ABIN7043324)
抗原
See all MC4R 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 6-20, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse - 13,15 amino acid residues identical
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产品特性
- Anti-MC4 Receptor (extracellular) Antibody (ABIN7043324, ABIN7044596 and ABIN7044597) is a highly specific antibody directed against an epitope of the human melanocortin receptor 4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It recognizes an extracellular epitope and can potentially recognize the receptor in living cells. It has been designed to recognize MC4R from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HRGMHTSLHLWNRSS, corresponding to amino acid residues 6-20 of human MC4R
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亚型
- IgG
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anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody
MC4R 适用: 人, 大鼠, 小鼠 ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (N-Term) antibodyMC4R 适用: 人, 猴, 马 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 25-57) antibodyMC4R 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB52063 unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 21-33) antibodyMC4R 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (Cytoplasmic Domain) antibodyMC4R 适用: 人, 绵羊, 兔, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (C-Term) antibodyMC4R 适用: 人, 大鼠, 小鼠, 绵羊, 兔, Cow, 猴, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 1-50) antibody (AbBy Fluor® 488)MC4R 适用: 人, 大鼠, 小鼠 IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Melanocortin 4 Receptor (MC4R) (N-Term) antibodyRecent MC4R 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Melanocortin 4 Receptor (MC4R) (AA 1-43) antibodyMC4R 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7235928)
Blocking Peptide: (ABIN7235928)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MC4R (Melanocortin 4 Receptor (MC4R))
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别名
- MC4R
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背景
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Melanocortin receptor 4, MC4R, MC4-R,Melanocortin receptor 4 (MC4R) is one of five members of the melanocortin receptor family, which belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.The ligands of these receptors, the melanocortins, are a group of structurally-related peptides comprising the α-, β-, and γ-melanocyte-stimulating hormone (α-, β-, γ-MSH) and the adrenocorticotropic hormone (ACTH), all of which are derived from post-translational processing of a common precursor peptide, proopiomelanocortin (POMC).1,2,3One of the salient features of the melanocortin signaling system is the existence of two endogenous antagonists, proteins that bind specifically to the receptor and have an inhibitory effect. These antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP).1All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti/ASP and AGRP) with differing affinities. The order of potency for MC4R activation is α-MSH = ACTH > β-MSH >> γ-MSH. AGRP and agouti/ASP are both endogenous antagonists of MC4R.1,2,3MC4R is widely expressed in the brain including the cortex, thalamus, hypothalamus, and brain stem. The best understood physiological role of MC4R is in the regulation of feeding behavior and the control of body weight. Indeed, mice with a targeted disruption of the MC4R gene display an obese phenotype characterized by increased adiposity, hyperphagia, and hyperleptinaemia.4 Similarly, MC4R mutations in humans have been associated with severe childhood obesity including characteristics very similar to the MC4R knockout mouse phenotype.5 All these make MC4R an attractive drug target for the treatment of obesity.
Alternative names: MC4 Receptor, Melanocortin receptor 4, MC4R, MC4-R -
基因ID
- 4160
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NCBI登录号
- NM_005912
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UniProt
- P32245
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途径
- Hormone Transport, cAMP Metabolic Process, Feeding Behaviour
抗原
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