Cytokeratin 7 抗体 (N-Term)
Quick Overview for Cytokeratin 7 抗体 (N-Term) (ABIN2746303)
抗原
See all Cytokeratin 7 (KRT7) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-60, N-Term
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交叉反应
- 绵羊, 猴, Panda (Ailuropoda melanoleuca)
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交叉反应 （详细）
- The antibody does not cross react to any other cellular protein.
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产品特性
- CK7- selective antibodies were generated against a peptide taken from the human protein. The CK7-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti- CK7-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. antibodies-online.com provides CK7 Western blot positive control samples in SDS-PAGE sample buffer.
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纯化方法
- Affinity Purified
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免疫原
- Synthetic peptide taken within amino acid region 10-60 on human keratin, type II cytoskeletal 7 protein.
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亚型
- IgG
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anti-Keratin 7 (KRT7) (AA 251-350) antibody
KRT7 适用: 人, 小鼠 WB, FACS, IHC (p), ELISA, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 91-394) antibodyKRT7 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 394-457) antibodyKRT7 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 1-94) antibodyKRT7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) (AA 2-469) antibodyKRT7 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal rOV-TL12-30 unconjugated Recombinant Antibody
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠, 小鼠 WB, IHC, IP 宿主: 小鼠 Monoclonal 12D7 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 WB, IHC, FACS, StM, ISt 宿主: 兔 Monoclonal KRT7-1499R unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT7-760-OV-TL12-30 unconjugated
anti-Keratin 7 (KRT7) antibodyKRT7 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal KRT7-903 unconjugated
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应用备注
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Antibodies were tested in ELISA and western blotting applications at 1:500 dilution using ABIN1686526 samples. Antibody dilutions for these antibodies are for reference only, investigators are expected to determine the optimal conditions. Application of this antibody in other protocols has not yet tested.
WB: > 1:500
IMM & IP pull-down assays: n.d.
IHC: n.d.
Investigators using this antibody in protocols other than listed above can request a complimentary sample of this antibody. n.d. not necessarily means the antibody is not suitable for that application, it simply means we have not yet characterized the antibody for that application.
The antibody labels a strong band of CK7 at 56 kDain ABIN1686526 samples and in other cancer cell lines. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.64-0.72 μg/μL
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储存条件
- -20 °C
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储存方法
- Storage of very dilute antibody solutions is not recommended.
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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别名
- Cytokeratin 7
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背景
- Cytokeratins (CKs, also called keratins) are part of the cytoskeleton family of intermediate filament-forming proteins (IFPs) that provide mechanical support and also provide a role in cell differentiation and tissue specialization to maintain cellular integrity. Cytokeratins are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85 % of mature keratinocytes in the vertebrate epidermis.Two types of Cytokeratins are known to exist, namely acidic type I (Cytokeratins 9-23) and basic type II (Cytokeratins 1-8). These Cytokeratins make homo and heterodimers that are specific for particular type of carcinoma. Motifs in certain Cytokeratins make them likely substrates for caspase degradation, and their subsequent release occurs during the intermediate events in apoptosis. Cytokeratin 7 which is a type II keratin (also known as sarcolectin) is one of the 20 different Cytokeratins identified and is expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II Cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants and antibodies to cytokeratin 7 are useful in the differential diagnosis of prostate carcinomas where CK7 is rarely expressed. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane. Expression of CK 7 is seen in majority of cases of cervical squamous cell carcinomas and is negative in other cases of carcinomas specially arising from the lung and GI tract.
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NCBI登录号
- NP_005547
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UniProt
- P08729
抗原
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