Poliovirus Receptor 抗体 (Biotin)
Quick Overview for Poliovirus Receptor 抗体 (Biotin) (ABIN2743906)
抗原
See all Poliovirus Receptor (PVR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.
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纯化方法
- Protein A purified
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免疫原
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Recombinant Human CD155.
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA, Flo
Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 μL of 300 ^g/mL human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD155/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- Poliovirus Receptor (PVR)
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别名
- PVR / CD155
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背景
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Name/Gene ID: PVR
Synonyms: PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4 -
基因ID
- 5817
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome
抗原
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