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Poliovirus Receptor 抗体 (Biotin)

This anti-Poliovirus Receptor antibody is a 小鼠 单克隆 antibody detecting Poliovirus Receptor in ELISA 和 FACS. Suitable for 人.
产品编号 ABIN2743906
发货至: 中国

Quick Overview for Poliovirus Receptor 抗体 (Biotin) (ABIN2743906)

抗原

See all Poliovirus Receptor (PVR) 抗体
Poliovirus Receptor (PVR)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Poliovirus Receptor antibody is conjugated to Biotin

应用范围

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ELISA, Flow Cytometry (FACS)

克隆位点

ANC2B2
  • 特异性

    Antibody ANC2B2 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

    纯化方法

    Protein A purified

    免疫原

    Recombinant Human CD155.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, Flo

    Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 μL of 300 ^g/mL human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-human CD155/Biotin at concentration of 10 ^g/mL. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.8 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 4°C, avoid freeze-thaw cycles.
  • 抗原

    Poliovirus Receptor (PVR)

    别名

    PVR / CD155

    背景

    Name/Gene ID: PVR

    Synonyms: PVR, CD155, Hpvr, HVED, Nectin-like 5, Nectin-like protein 5, Poliovirus receptors beta, Poliovirus receptor, PVS, Necl-5, CD155 antigen, NECL5, TAGE4

    基因ID

    5817

    途径

    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome
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