NGFR 抗体 (Biotin)
Quick Overview for NGFR 抗体 (Biotin) (ABIN2743244)
抗原
See all NGFR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.
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纯化方法
- Protein A purified
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免疫原
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Recombinant soluble human CD271.
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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anti-Nerve Growth Factor Receptor (NGFR) (AA 301-400) antibody
NGFR 适用: 人, 大鼠, 小鼠 WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 大鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) (AA 281-421) antibodyNGFR 适用: 人 IHC, Diff, StM 宿主: 小鼠 Monoclonal NGFR-1964 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) (AA 188-203), (Extracellular) antibodyKO Validated NGFR 适用: 人 WB, FACS, IHC, IF, IP, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, Non-Human Primate IHC, Diff, StM 宿主: 兔 Monoclonal NGFR-1997R unconjugated Recombinant Antibody
anti-Nerve Growth Factor Receptor (NGFR) (AA 281-421) antibodyNGFR 适用: 人 IHC, Diff, StM 宿主: 小鼠 Monoclonal rNGFR-1965 unconjugated Recombinant Antibody
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, Non-Human Primate, 兔, Cat, 雪貂 WB, FACS, IHC (p), IP, ICC, ICFC, MS 宿主: 小鼠 Monoclonal NGFR5 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2F1C2 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, 小鼠 WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 8G3G10 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 8G3G10 unconjugated
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应用备注
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Approved: ELISA, Flo
Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-CD271/Biotin at a concentration of 10 μg/mL. Cells were then washed twice and incubated with secondary reagent Streptaviden/R-PE. They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.17 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- NGFR (Nerve Growth Factor Receptor (NGFR))
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别名
- NGFR / CD271
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背景
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Name/Gene ID: NGFR
Family: TNF Receptor
Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16 -
基因ID
- 4804
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途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
抗原
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