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NGFR 抗体 (FITC)

The FITC-conjugated 小鼠 单克隆 anti-NGFR antibody (Clone ANC271-3D7) (ABIN2743242) specifically detects NGFR in FACS 和 ELISA. The antibody is reactive with 人 samples.
产品编号 ABIN2743242
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for NGFR 抗体 (FITC) (ABIN2743242)

抗原

See all NGFR 抗体
NGFR (Nerve Growth Factor Receptor (NGFR))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This NGFR antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), ELISA

克隆位点

ANC271-3D7
  • 特异性

    Antibody ANC271/3D7 binds to recombinant CD271-muIg in EIA, and to endogenous CD271 on cell surface of human Reh cells.

    纯化方法

    Protein A purified

    免疫原

    Recombinant soluble human CD271.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, Flo

    Usage: Five x 105 cultured human SW480 cells were harvested by EDTA, washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of product at a 1:50 dilution (10 μg/mL). They were then washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.55 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD271 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles

    储存条件

    4 °C

    储存方法

    Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
  • 抗原

    NGFR (Nerve Growth Factor Receptor (NGFR))

    别名

    NGFR / CD271

    背景

    Name/Gene ID: NGFR
    Family: TNF Receptor

    Synonyms: NGFR, CD271, CD271 antigen, gp80-LNGFR, Nerve growth factor receptor, p75 ICD, p75(NTR), p75NTR, TNFR superfamily, member 16, NGF receptor, TNFRSF16

    基因ID

    4804

    途径

    NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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