LRRC32 抗体
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- 抗原 See all LRRC32 抗体
- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LRRC32 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), ELISA
- 特异性
- Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
- 纯化方法
- Protein A purified
- 免疫原
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Recombinant human GARP.
Type of Immunogen: Recombinant protein - 克隆位点
- ANC8C9
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product LRRC32 Primary Antibody
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anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 162-405) antibody
LRRC32 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 234-260) antibodyLRRC32 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 234-260) antibodyLRRC32 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB18218 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 400-643) antibodyLRRC32 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) antibodyLRRC32 适用: 人 WB 宿主: 小鼠 Monoclonal GARP5 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 250-300) antibodyLRRC32 适用: 人, 小鼠, 大鼠, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 301-500) antibodyLRRC32 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 23-612) antibodyLRRC32 适用: 人 WB 宿主: 小鼠 Monoclonal 50G10 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 240-269) antibodyLRRC32 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, Flo
Usage: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- 抗原
- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
- 别名
- LRRC32 (LRRC32 产品)
- 背景
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Name/Gene ID: LRRC32
Synonyms: LRRC32, D11S833E, GARP, Garpin - 基因ID
- 2615
- 途径
- Activated T Cell Proliferation
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