LRRC32 抗体
Quick Overview for LRRC32 抗体 (ABIN2742631)
抗原
See all LRRC32 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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                                            特异性
- Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
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                                            纯化方法
- Protein A purified
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                                            免疫原
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                        Recombinant human GARP.
 Type of Immunogen: Recombinant protein
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                                            亚型
- IgG2a kappa
 
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                                            应用备注
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                        Approved: ELISA, Flo
 Usage: (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate and anti-human GARP/Biotin at a concentration of 5 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 4.7 % sub population of gated cells stained positive with a mean shift of 1.59 log10 fluorescent units when compared to a Mouse IgG2a/Biotin isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb.
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                                            说明
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                        Target Species of Antibody: Human 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- Lot specific
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                                            缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.
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                                            储存液
- Gentamicin sulfate
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                                            注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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                                            储存条件
- 4 °C
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                                            储存方法
- Store at 4°C, avoid freeze-thaw cycles.
 
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    - LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
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                                            别名
- LRRC32
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                                            背景
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                        Name/Gene ID: LRRC32
 Synonyms: LRRC32, D11S833E, GARP, Garpin
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                                            基因ID
- 2615
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                                            途径
- Activated T Cell Proliferation
 抗原
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