LRRC32 抗体 (FITC)
Quick Overview for LRRC32 抗体 (FITC) (ABIN2742629)
抗原
See all LRRC32 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
-
纯化方法
- Protein A purified
-
免疫原
-
Recombinant human GARP.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a kappa
-
-
-
-
应用备注
-
Approved: ELISA, Flo
Usage: : (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 μg/mL). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1 % sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- avoid freeze thaw cycles
-
储存条件
- 4 °C
-
储存方法
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
-
-
- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
-
别名
- LRRC32
-
背景
-
Name/Gene ID: LRRC32
Synonyms: LRRC32, D11S833E, GARP, Garpin -
基因ID
- 2615
-
途径
- Activated T Cell Proliferation
抗原
-