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LRRC32 抗体 (FITC)

This anti-LRRC32 antibody is a 小鼠 单克隆 antibody detecting LRRC32 in FACS 和 ELISA. Suitable for 人.
产品编号 ABIN2742629
发货至: 中国

Quick Overview for LRRC32 抗体 (FITC) (ABIN2742629)

抗原

See all LRRC32 抗体
LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

适用

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宿主

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小鼠

克隆类型

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  • 11
单克隆

标记

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This LRRC32 antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), ELISA

克隆位点

ANC8C9
  • 特异性

    Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.

    纯化方法

    Protein A purified

    免疫原

    Recombinant human GARP.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a kappa
  • 应用备注

    Approved: ELISA, Flo

    Usage: : (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 μg/mL). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1 % sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles

    储存条件

    4 °C

    储存方法

    Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
  • 抗原

    LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))

    别名

    LRRC32

    背景

    Name/Gene ID: LRRC32

    Synonyms: LRRC32, D11S833E, GARP, Garpin

    基因ID

    2615

    途径

    Activated T Cell Proliferation
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