LRRC32 抗体 (FITC)
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北京 101111
Quick Overview for LRRC32 抗体 (FITC) (ABIN2742629)
抗原
See all LRRC32 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody ANC8C9 binds to recombinant GARP in EIA and to cell surface GARP expressed on a subset of FOXP3 positive cells.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human GARP.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a kappa
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anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 162-405) antibody
LRRC32 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 234-260) antibodyLRRC32 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 234-260) antibodyLRRC32 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB18218 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 400-643) antibodyLRRC32 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) antibodyLRRC32 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal GARP5 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 250-300) antibodyLRRC32 适用: 人, 小鼠, 大鼠, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 301-500) antibodyLRRC32 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 23-612) antibodyLRRC32 适用: 人 WB 宿主: 小鼠 Monoclonal 50G10 unconjugated
anti-Leucine Rich Repeat Containing 32 (LRRC32) (AA 240-269) antibodyLRRC32 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Flo
Usage: : (FACS analysis) Ficoll prepared human PBMC were stimulated in culture overnight with immobilized anti-human CD3 antibody, anti-human CD28 mAb, and human IL-2. Cells were harvested and 5 x 105 cells per tube were washed and pre incubated ~5 minutes with 20 μL human IgG at 250 μg/mL (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 μL of anti-CD4/APC conjugate, and anti-human GARP/FITC at dilution factor of 1:50 (5 μg/mL). Cells were washed twice, fixed, permeabolized and co stained with anti-FOXP3/PE after which they were washed twice and analyzed by FACS, gating on CD4 positive lymphoid cells. A net 2.1 % sub population of FOXP3 positive cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG2a/FITC isotype control. This binding was blocked when cells were pre incubated with 50 μg/mL unlabeled anti-human GARP mAb. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- LRRC32 (Leucine Rich Repeat Containing 32 (LRRC32))
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别名
- LRRC32
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背景
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Name/Gene ID: LRRC32
Synonyms: LRRC32, D11S833E, GARP, Garpin -
基因ID
- 2615
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途径
- Activated T Cell Proliferation
抗原
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