CEACAM8 抗体 (Biotin)
Quick Overview for CEACAM8 抗体 (Biotin) (ABIN2739905)
抗原
See all CEACAM8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody from clone ANC1D5 binds to CD66b on the cell surface of human PMN in Flow cytometry and detects recombinant CD66b in sandwich EIA utilizing either clone ANC4A4 or ANC3C5 as a capture reagent.
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纯化方法
- Protein A purified
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免疫原
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Recombinant soluble human CD66b.
Type of Immunogen: Recombinant protein -
亚型
- IgG1
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应用备注
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Approved: ELISA, Flo
Usage: Five x 105 Polymorphprep prepared human Granulocytes(PMN) per tube were pelleted and pre incubated 5 minutes with 20 μL of human IgG at 300 μg/mL (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 μL of anti-CD66b antibody(ANC1D5) at 10 μg/mL. Cells were washed twice and incubated with 50 μL of 2o reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.66 log10 fluorescent units when compared to a Mouse IgG1/Biotin isotype control. Binding was blocked when cells were pre incubated with an excess of unlabeled anti-human CD66b (ANC1D5) mAb. ELISA: Use for detection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 4°C, avoid freeze-thaw cycles.
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- CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))
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别名
- CEACAM8 / CD66b
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背景
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Name/Gene ID: CEACAM8
Family: CEA
Synonyms: CEACAM8, CD66b antigen, Carcinoembryonic antigen CGM6, CD67, CD67 antigen, CGM6, NCA-95, CD66b -
基因ID
- 1088
抗原
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