CD8 抗体 (Cy7,PE)
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北京 101111
Quick Overview for CD8 抗体 (Cy7,PE) (ABIN2739653)
抗原
See all CD8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody 14 recognizes the CD8 alpha chain of approximately 33 kd (1).
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纯化方法
- Protein A purified
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免疫原
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Mouse IgG1, kappa.
Type of Immunogen: Purified protein -
亚型
- IgG1 kappa
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) (AA 135-235) antibodyCD8 适用: 人 IHC 宿主: 兔 Monoclonal 008R unconjugated Recombinant Antibody
anti-CD8 (CD8) antibodyCD8 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (Biotin)CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PE)CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE
anti-CD8 (CD8) antibody (APC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC
anti-CD8 (CD8) antibody (PerCP)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP
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应用备注
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Approved: ELISA, Flo
Usage: Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 μL of 300 μg/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of antihuman CD8/PE-Cy7 at a 1:50 dilution (5 μg/mL). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 25.7 % sub population of the cells stained positive with a mean shift of 2.5 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-human CD8 antibody. ELISA: Use for detection. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Do not freeze. Protect from light.
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- CD8
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别名
- CD8
抗原
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