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CD3 epsilon 抗体

This anti-CD3 epsilon antibody is a 仓鼠 单克隆 antibody detecting CD3 epsilon in FACS, WB, IP 和 ELISA. Suitable for 小鼠.
产品编号 ABIN2739340
发货至: 中国
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Quick Overview for CD3 epsilon 抗体 (ABIN2739340)

抗原

See all CD3 epsilon (CD3E) 抗体
CD3 epsilon (CD3E)

适用

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小鼠

宿主

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仓鼠

克隆类型

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单克隆

标记

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This CD3 epsilon antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA

克隆位点

145-2C11
  • 特异性

    Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.

    纯化方法

    Protein G purified

    免疫原

    Mouse BM10-37 T cell membrane prep.

    Type of Immunogen: Membrane preparation

    亚型

    IgG
  • 应用备注

    Approved: ELISA, Flo, IP, WB

    Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL murine IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 μL of anti-CD3 antibody diluted to 10 μg/mL. Cells were washed twice and incubated with 2o reagent Goat anti-Hampster IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 33 % sub population of the cells stained positive with a mean shift of 1.08 log10 fluorescent units when compared to background.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    50 mM sodium phosphate,  pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/mL Gentamicin sulfate.

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 4°C, avoid freeze-thaw cycles.
  • 抗原

    CD3 epsilon (CD3E)

    别名

    CD3E

    背景

    Name/Gene ID: CD3E

    Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

    基因ID

    916

    途径

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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