CD3 epsilon 抗体 (FITC)
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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适用
- 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This CD3 epsilon antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 特异性
- Antibody 145-2C11binds to an extracellular epitope of the 25 kd CD3e molecule present on murine T cells.
- 纯化方法
- Protein G purified
- 免疫原
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Mouse BM10-37 T cell membrane prep.
Type of Immunogen: Membrane preparation - 克隆位点
- 145-2C11
- 亚型
- IgG
- Top Product
- Discover our top product CD3E Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, IP, WB
Usage: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-mouse CD3/FITC at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. A 35 % sub population of the cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD3 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 5 % glycerol
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- avoid freeze thaw cycles
- 储存条件
- 4 °C
- 储存方法
- Store at 4°C. Avoid freeze-thaw cycles and prolonged exposure to light.
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 别名
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- 背景
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Name/Gene ID: CD3E
Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon - 基因ID
- 916
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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