PD-L1 抗体 (PE)
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北京 101111
Quick Overview for PD-L1 抗体 (PE) (ABIN2738460)
抗原
See all PD-L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human CD274(B7-H1).
Type of Immunogen: Recombinant protein -
亚型
- IgG1 kappa
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anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 19-238) antibodyPD-L1 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 9B5F4 unconjugated
anti-CD274 (PD-L1) (AA 39-191) antibodyPD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated
anti-CD274 (PD-L1) (AA 48-290) antibodyPD-L1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 34-241) antibodyPD-L1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (C-Term) antibodyKO Validated PD-L1 适用: 人 WB, FACS, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) (AA 24-153) antibodyPD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated
anti-CD274 (PD-L1) (AA 24-234) antibodyPD-L1 适用: 人 WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD274 (PD-L1) antibodyPD-L1 适用: 人 IHC 宿主: 兔 Monoclonal 711R unconjugated Recombinant Antibody
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应用备注
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Approved: ELISA, Flo
Usage: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 μg/mL PHA. 5 x 105 per tube were washed and pre incubated 5 minutes with 20^l of 250^g/mL human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-human CD274 at a dilution factor of 1:50 (20 ^g/mL). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 50 mM sodium phosphate, pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Do not freeze. Protect from light.
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- PD-L1 (CD274 (PD-L1))
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别名
- B7-H1 / PD-L1 / CD274
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背景
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Name/Gene ID: CD274
Synonyms: CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 Molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1 -
基因ID
- 29126
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途径
- Cancer Immune Checkpoints
抗原
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