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LGR5 抗体 (AA 250-550)

Name: LGR5 抗体 (AA 250-550)
SKU: ABIN2724692
Availability: OutOfStock

This anti-LGR5 antibody is a 小鼠单克隆 antibody detecting LGR5 in WB, FACS, IHC, IF 和 IP. Suitable for 人和小鼠. This Primary Antibody has been cited in 16+ publications.

HEK293T cells were transfected with the empty vector control (Left lane) or Human LGR5 expression plasmid (Right lane) cDNA for 48 hrs and lysed. (LGR5 抗体 (AA 250-550))

Anti-LGR5 mouse monoclonal antibody ABIN2454875 immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5 (RC212845). (LGR5 抗体 (AA 250-550))

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-LGR5 mouse monoclonal antibody. (LGR5 抗体 (AA 250-550))

IHC

3 images

(16 references)

产品编号 ABIN2724692

发货至: 中国

Quick Overview for LGR5 抗体 (AA 250-550) (ABIN2724692)

抗原

See all LGR5 抗体

LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This LGR5 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

克隆位点

2A2

Discover our top product LGR5 Primary Antibody

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AA 250-550

产品特性

Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)

纯化方法

Purified from mouse ascites fluids by affinity chromatography

免疫原

Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.

亚型

IgG1
anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 810-907) antibody
LGR5 适用: 人, 小鼠 WB, FACS, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473) antibody
LGR5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14555 unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 22-178) antibody
LGR5 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 2B5B9 unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 443-473) antibody
LGR5 适用: 人 WB, FACS, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719) antibody
LGR5 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (Cytoplasmic Domain) antibody
LGR5 适用: 人 IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 824-907) antibody
LGR5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 241-352) antibody
LGR5 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) antibody
LGR5 适用: 人 WB, FACS, ELISA 宿主: 兔 Monoclonal 4H8 unconjugated Recombinant Antibody

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (N-Term) antibody
LGR5 适用: 人, Bat, 猴 IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2 μg/500 μL

说明

The concentration of the product may vary between diferrent lots.

限制

仅限研究用
状态

Liquid

浓度

0.5-1.0 mg/mL

缓冲液

PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Patel, Sharon, Baranwal, Boothello, Desai, Patel: "Heparan sulfate hexasaccharide selectively inhibits cancer stem cells self-renewal by activating p38 MAP kinase." in: Oncotarget, Vol. 7, Issue 51, pp. 84608-84622, (2018) (PubMed).

Yang, Bavi, Wang, Roehrl: "Immuno-proteomic discovery of tumor tissue autoantigens identifies olfactomedin 4, CD11b, and integrin alpha-2 as markers of colorectal cancer with liver metastases." in: Journal of proteomics, Vol. 168, pp. 53-65, (2018) (PubMed).

Miyake, Li, Lee, Koike, Chen, Seo, Pierro: "Liver damage, proliferation, and progenitor cell markers in experimental necrotizing enterocolitis." in: Journal of pediatric surgery, Vol. 53, Issue 5, pp. 909-913, (2018) (PubMed).

Ma, Wang, Wang, Luo, Fan: "NANOGP8 is the key regulator of stemness, EMT, Wnt pathway, chemoresistance, and other malignant phenotypes in gastric cancer cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0192436, (2018) (PubMed).

Liu, Zheng, Ji, Liu, Hou, Li, Li: "Resveratrol reduces senescence-associated secretory phenotype by SIRT1/NF-κB pathway in gut of the annual fish Nothobranchius guentheri." in: Fish & shellfish immunology, Vol. 80, pp. 473-479, (2018) (PubMed).

Wang, Chen, Cao, Ma, Yin, Jia, Zang, Fan: "LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1." in: PLoS ONE, Vol. 11, Issue 12, pp. e0168904, (2017) (PubMed).

Osawa, Takahashi, Nishimura, Ohta, Haraguchi, Hata, Yamamoto, Mizushima, Takemasa, Doki, Mori: "Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer." in: British journal of cancer, Vol. 114, Issue 11, pp. 1251-60, (2016) (PubMed).

Chen, Wei, Sun, Bhattacharya, Savage, Serda, Mackeyev, Curley, Bu, Wang, Chen, Cohen-Gould, Huang, Shen, Lipkin, Copeland, Jenkins, Shuler: "A recellularized human colon model identifies cancer driver genes." in: Nature biotechnology, Vol. 34, Issue 8, pp. 845-51, (2016) (PubMed).

Hsu, Liu, Tseng, Yang, Yeh, You, Hung, Chen, Chen: "CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 1, pp. 11-21, (2015) (PubMed).

Shaikh, Zhou, Teo, Garg, Neogi, Figg, Yeo, Yu, Maguire, Zhao, Bennett, Azizan, Davenport, McKenzie, Brown: "LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 6, pp. E836-44, (2015) (PubMed).

Jiang, Zhou, Chen, Schiff, Lee, Kong, Embree, Zhou, Mao: "Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis." in: Biomaterials, Vol. 35, Issue 7, pp. 2172-80, (2014) (PubMed).

Wang, Guo, Lui, Chu, Yoo, Chang, Yen: "Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro." in: PLoS ONE, Vol. 9, Issue 1, pp. e85112, (2014) (PubMed).

Hsu, Liu, Tseng, Tan, Chen, Chen: "LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/?-catenin signaling pathway in colorectal cancer cells." in: Cellular signalling, Vol. 26, Issue 11, pp. 2333-42, (2014) (PubMed).

Lühnsdorf, Epe, Khobta: "Excision of uracil from transcribed DNA negatively affects gene expression." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22008-18, (2014) (PubMed).

Wu, Xie, Gao, Wang, Guo, Tian, Li, Fan: "Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers." in: Gene, Vol. 525, Issue 1, pp. 18-25, (2013) (PubMed).

Pastò, Marchesi, Diamantini, Frasson, Curtarello, Lago, Pilotto, Parenti, Esposito, Agostini, Nitti, Amadori: "PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages." in: PLoS ONE, Vol. 7, Issue 8, pp. e43379, (2012) (PubMed).
抗原

LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

别名

LGR5

背景

LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer, overexpressed in ovary and liver tumors.

分子量

99.8 kDa

基因ID

8549

NCBI登录号

NM_003667

HGNC

8549

途径

WNT signaling