LIG4 抗体
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北京 101111
Quick Overview for LIG4 抗体 (ABIN272301)
抗原
See all LIG4 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of DNA Ligase IV protein. (region surrounding Gly619)
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity Chromatography using epitope-specific immunogen
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anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibody
KD Validated LIG4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB2515 unconjugated Recombinant Antibody
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (C-Term) antibodyLIG4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 612-911) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 802-911) antibodyLIG4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D2 unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (N-Term) antibodyLIG4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 275-324) antibodyLIG4 适用: 人, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) antibodyKO Validated LIG4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (Center) antibodyLIG4 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase IV, DNA, ATP-Dependent (LIG4) (AA 1-911) antibodyLIG4 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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ELISA: 1/20000-1/40000. Western Blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))
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别名
- DNA Ligase 4
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背景
- The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These proteins form a critical complex consisting of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligate accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4/ DNA Ligase IV complex is also involved in V(D)J recombination. V(D)J recombination occurs in normal development of the adaptive immune system and involves the formation of a double-strand break intermediate. Deletions of either DNA Ligase IV or XRCC4 inhibit the completion of V(D)J recombination, resulting in a high incidence of apoptosis in the developing nervous system and a block in B and T cell maturation.Synonyms: DNA ligase IV, EC=6.5.1.1, LIG4, Polydeoxyribonucleotide synthase [ATP] 4
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分子量
- 104 kDa
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基因ID
- 3981
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NCBI登录号
- NP_001091738
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UniProt
- P49917
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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