DFFA 抗体
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- 抗原 See all DFFA 抗体
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DFFA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects endogenous levels of ICAD protein. (region surrounding Gln177)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product DFFA Primary Antibody
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anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibody
DFFA 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 51-305) antibodyDFFA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 231-331) antibodyDFFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) antibodyDFFA 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 2-21) antibodyDFFA 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 179-331) antibodyDFFA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2B10C8 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 19-262) antibodyDFFA 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) antibodyDFFA 适用: 人, 猴 WB, IF 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (C-Term) antibodyDFFA 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- DO NOT FREEZE!
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
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- 抗原
- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
- 别名
- DFFA / ICAD (DFFA 产品)
- 背景
- The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD
- 分子量
- approx. 36 kDa
- 基因ID
- 13347
- NCBI登录号
- NP_034174
- UniProt
- O54786
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
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