NFIA 抗体
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北京 101111
Quick Overview for NFIA 抗体 (ABIN272219)
抗原
See all NFIA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of NF-1 protein. (region surrounding Gln34)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibody
NFIA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibodyNFIA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-224), (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 348-397) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, Pig, 豚鼠, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibodyNFIA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 384-418), (C-Term) antibodyNFIA 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51284 unconjugated
anti-Nuclear Factor I/A (NFIA) (C-Term) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-143) antibodyNFIA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- NFIA (Nuclear Factor I/A (NFIA))
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别名
- NFIA
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背景
- NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.Synonyms: CCAAT-box-binding transcription factor, CTG, NF-I/A, NFI-A, Nuclear factor 1 A-type, TGGCA-binding protein
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分子量
- approx. 55 kDa
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基因ID
- 4774
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NCBI登录号
- NP_001128145
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UniProt
- Q12857
抗原
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