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NFIA 抗体

This anti-NFIA antibody is a 兔 多克隆 antibody detecting NFIA in WB 和 EIA. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN272219
发货至: 中国

Quick Overview for NFIA 抗体 (ABIN272219)

抗原

See all NFIA 抗体
NFIA (Nuclear Factor I/A (NFIA))

适用

  • 47
  • 20
  • 19
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
人, 小鼠, 大鼠

宿主

  • 41
  • 6

克隆类型

  • 41
  • 6
多克隆

标记

  • 35
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NFIA antibody is un-conjugated

应用范围

  • 40
  • 14
  • 10
  • 8
  • 6
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    This antibody detects endogenous levels of NF-1 protein. (region surrounding Gln34)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    纯度

    > 95 % pure by SDS-PAGE
  • 应用备注

    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1,0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    DO NOT FREEZE!

    储存条件

    4 °C

    储存方法

    Store the antibody undiluted at 2-8 °C.
  • 抗原

    NFIA (Nuclear Factor I/A (NFIA))

    别名

    NFIA

    背景

    NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.Synonyms: CCAAT-box-binding transcription factor, CTG, NF-I/A, NFI-A, Nuclear factor 1 A-type, TGGCA-binding protein

    分子量

    approx. 55 kDa

    基因ID

    4774

    NCBI登录号

    NP_001128145

    UniProt

    Q12857
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