Histone H3.3 抗体
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Quick Overview for Histone H3.3 抗体 (ABIN272113)
抗原
See all Histone H3.3 (H3F3A) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Histone H3.3 protein. (region surrounding Lys27)
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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应用备注
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ELISA: 1: 5000approx. 1: 10000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- Histone H3.3
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背景
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones.Synonyms: H3.3A, H3F3, H3F3A, PP781
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基因ID
- 3020, 9606
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UniProt
- P84243
抗原
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