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Histone H3.3 抗体

This 兔 多克隆 antibody specifically detects Histone H3.3 in IF 和 IHC (p). It exhibits reactivity toward 人, 小鼠 和 大鼠.
产品编号 ABIN272113
发货至: 中国
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Quick Overview for Histone H3.3 抗体 (ABIN272113)

抗原

See all Histone H3.3 (H3F3A) 抗体
Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

适用

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人, 小鼠, 大鼠

宿主

  • 95
  • 13

克隆类型

  • 57
  • 51
多克隆

标记

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This Histone H3.3 antibody is un-conjugated

应用范围

  • 75
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Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of Histone H3.3 protein. (region surrounding Lys27)

    交叉反应 (详细)

    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    纯度

    > 95 % pure by SDS-PAGE
  • 应用备注

    ELISA: 1: 5000approx. 1: 10000. IHC: 1: 50approx. 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1,0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    DO NOT FREEZE!

    储存条件

    4 °C

    储存方法

    Store the antibody undiluted at 2-8 °C.
  • 抗原

    Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

    别名

    Histone H3.3

    背景

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones.Synonyms: H3.3A, H3F3, H3F3A, PP781

    基因ID

    3020, 9606

    UniProt

    P84243
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