SP1 抗体
Quick Overview for SP1 抗体 (ABIN272083)
抗原
See all SP1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of SP1 protein. (region surrounding Gly447)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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纯度
- > 95 % pure by SDS-PAGE
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anti-Sp1 Transcription Factor (SP1) (pThr453) antibody
SP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (C-Term) antibodySP1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibodySP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 522-618) antibodySP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B11 unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-198) antibodySP1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A5 unconjugated
anti-Sp1 Transcription Factor (SP1) antibodySP1 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 89-199) antibodySP1 适用: 人, 小鼠 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4H6 unconjugated
anti-Sp1 Transcription Factor (SP1) (pThr739) antibodySP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 420-620) antibodySP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sp1 Transcription Factor (SP1) (AA 677-707), (C-Term) antibodySP1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB17350 unconjugated
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应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should bedetermined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE!
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- SP1 (Sp1 Transcription Factor (SP1))
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背景
- Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences. Evidence suggests that the early growth response gene, Erg-1 (also known as Zif268 or NGF1-A), may downregulate certain mammalian gene promoters by competing with Sp1 for binding to an overlapping binding motif. The gene encoding human Sp1 maps to chromosome 12q13.1.Synonyms: Transcription factor Sp1
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分子量
- approx. 90 kDa
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基因ID
- 6667
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NCBI登录号
- NP_003100
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UniProt
- P08047
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途径
- Regulation of Lipid Metabolism by PPARalpha, Myometrial Relaxation and Contraction
抗原
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