ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15,17) (q22,q11.2-q12) chromosomal trans-location of the retinoic acid receptor alpha (RARalpha) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARalpha chimeric protein. Myeloid precursor cells expressing the PML/ RARalpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARalpha transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARalpha also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.Synonyms: MYL, Probable transcription factor PML, RING finger protein 71, TRIM19, Tripartite motif-containing protein 19