PML 抗体
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北京 101111
Quick Overview for PML 抗体 (ABIN271787)
抗原
See all PML 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of PML protein. (region surrounding Pro39)
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Affinity-chromatography using epitope-specific immunogen.
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anti-Promyelocytic Leukemia (PML) (AA 411-510) antibody
PML 适用: 人 WB, IF, ELISA, PLA 宿主: 小鼠 Monoclonal 1D12 unconjugated
anti-Promyelocytic Leukemia (PML) (N-Term) antibodyPML 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) (AA 141-179), (N-Term) antibodyPML 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) (AA 470-499), (Sumoylation Site) antibodyPML 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) (AA 300-600) antibodyPML 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) (AA 470-499), (Sumoylation Site) antibodyPML 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB2570 unconjugated
anti-Promyelocytic Leukemia (PML) (C-Term) antibodyPML 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 犬, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) (AA 411-510) antibodyPML 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2B10 unconjugated
anti-Promyelocytic Leukemia (PML) (C-Term) antibodyPML 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Promyelocytic Leukemia (PML) antibodyKD Validated PML 适用: 人 WB, IF (p) 宿主: 小鼠 Monoclonal 25GB8165 unconjugated
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应用备注
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ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PML (Promyelocytic Leukemia (PML))
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别名
- PML / RNF71
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背景
- The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15,17) (q22,q11.2-q12) chromosomal trans-location of the retinoic acid receptor alpha (RARalpha) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARalpha chimeric protein. Myeloid precursor cells expressing the PML/ RARalpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARalpha transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARalpha also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.Synonyms: MYL, Probable transcription factor PML, RING finger protein 71, TRIM19, Tripartite motif-containing protein 19
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分子量
- approx. 98 kDa
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基因ID
- 5371
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NCBI登录号
- NP_002666
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UniProt
- P29590
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途径
- p53 Pathway, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus
抗原
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